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Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia
https://asahi-u.repo.nii.ac.jp/records/11433
https://asahi-u.repo.nii.ac.jp/records/11433ce9d6657-07c9-4abd-aa23-61cb24af5d9c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
00029440_189_15261535_2019 (3.3 MB)
Restricted Access
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-22 | |||||
| タイトル | ||||||
| タイトル | Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia | |||||
| タイトル | ||||||
| タイトル | Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Suzuki, Kodai
× Suzuki, Kodai× Okada, Hideshi× 竹村, 元三× Takada, Chihiro× Kuroda, Ayumi× Yano, Hirohisa× Zaikokuji, Ryogen× Morishita, Kentaro× Tomita, Hiroyuki× Oda, Kazumasa× Matsuo, Saori× Uchida, Akihiro× Fukuta, Tetsuya× Sampei, So× Miyazaki, Nagisa× Kawaguchi, Tomonori× Watanabe, Takatomo× Yoshida, Takahiro× Ushikoshi, Hiroaki× Yoshida, Shozo× Maekawa, Yoichi× Ogura, Shinji |
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| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Internal Medicine, Asahi University School of Dentistry | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine Laboratory of Molecular Biology, Department of Biofunctional Analysis, Gifu Pharmaceutical University |
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| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Tumor Pathology, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Internal Medicine, Asahi University School of Dentistry | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Clinical Laboratory, Gifu University Hospital | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 所属 | ||||||
| Department of Parasitology and Infectious Diseases, Gifu University Graduate School of Medicine Domain of Integrated Life Systems, Center for Highly Advanced Integration of Nano and Life Sciences (G-CHAIN), Gifu University |
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| 所属 | ||||||
| Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine | ||||||
| 書誌情報 |
en : The American journal of pathology 巻 189, 号 8, p. 1526-1535, 発行日 2019-08 |
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| 出版者 | ||||||
| Elsevier | ||||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Neutrophil elastase (NE) is necessary for effective sterilization of phagocytosed bacterial and fungal pathogens; however, NE increases alveolocapillary permeability and induces proinflammatory cytokine production in sepsis-induced acute respiratory distress syndrome. Under septic conditions, the pulmonary endothelial glycocalyx covering on the healthy endothelium surface is injured, but the contribution of NE to this injury remains unknown. Our aim was to examine whether NE-induced pulmonary endothelial injury is associated with endotoxemia. Lipopolysaccharide (LPS; 20 mg/kg) was injected intraperitoneally into 9- to 12-week-old granulocyte colony-stimulating factor knockout (G-CSFKO) mice, which harbor few neutrophils, and littermate control mice; in a second assay, mice were injected with the NE-inhibitor sivelestat (0.2 mg/kg) at 3, 6, 9, and 12 hours after LPS administration. Subsequently, vascular endothelial injury was evaluated through ultrastructural analysis. At 48 hours after LPS injection, survival rate was more than threefold higher among G-CSFKO than control mice, and degradation of both thrombomodulin and syndecan-1 was markedly attenuated in G-CSFKO compared with control mice. Ultrastructural analysis revealed attenuated vascular endothelial injury and clear preservation of the endothelial glycocalyx in G-CSFKO mice. Moreover, after LPS exposure, survival rate was approximately ninefold higher among sivelestat-injected mice than control mice, and sivelestat treatment potently preserved vascular endothelial structures and the endothelial glycocalyx. In conclusion, NE is associated with pulmonary endothelial injury under LPS-induced endotoxemic conditions. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1525-2191 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 31108101 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.ajpath.2019.05.002 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0002944018309933?via%3Dihub | |||||
| 関連名称 | 出版社サイト | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 見出し | ||||||
| 大見出し | Open Access | |||||
| 言語 | ja | |||||
