{"created":"2023-06-23T12:20:45.606496+00:00","id":12185,"links":{},"metadata":{"_buckets":{"deposit":"599de5ac-6c4d-495e-ba04-533fcdc723b4"},"_deposit":{"created_by":21,"id":"12185","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"12185"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00012185","sets":["46:348:446"]},"author_link":["15386","15384","15387","3809","3902","1290","15385"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"39","bibliographicPageStart":"27","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CCIラットは、全身性に長管骨の低成長など骨格的な形態異常を示す。これまでの兄妹交配ではCCIラットが約25％の頻度で誕生することから、常染色体劣性遺伝性疾患である可能性が予測されたが、具体的な遺伝様式や病態解明には至っていない。そこで、軟骨内骨化を示す頭蓋底軟骨結合に着目し、SDラットとCCIラットを形態学的ならびに分子生物学的に比較検討した。マイクロCT画像、HE染色およびSOFG染色の結果から、軟骨幅が増大し骨化が遅延することが低成長に繋がると考えられた。BMD値、DMP1のリアルタイムPCRやISHの結果から、骨密度や骨成熟についてはSDラットとCCIラット間に差がないと考えられた。BrdU免疫染色から、CCIラットでは軟骨細胞の過剰な増殖が認められた。またGli1の発現分布を広範囲に認めることから、成長板軟骨における軟骨細胞の成熟異常が示唆された。しかし、Ihhの発現には差が認められないことから、CCIラットの骨化は、Ihhシグナル系による骨化制御とは異なり、独立したGli1が関連するシグナル系の異常である可能性が高いと考えられた。さらに、DMP1の発現減少がCCIラットの肥大層軟骨細胞で認められることから、CCIラットでは肥大層軟骨細胞のアポトーシスの異常が生じ、肥大層の幅が広がった可能性が高いと考えられる。CCIラットの形態異常は軟骨細胞の過剰増殖と肥大層軟骨細胞の未成熟によるもので、その発症機序には、Gli1やDMP1の発現と分布異常が関係している可能性が示唆された。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"CCI rats show skeletal morphological abnormality such as low growth of long tubular bone systemically. Since CCI rats are born at a frequency of about 25% in previous brother-sister breeding, it was dicted that it may be an autosomal recessive inherited disorder. However, specific genetic modalities and pathological conditions have not been elucidated. Therefore, I focused on cranial base synchondrosis showing intra-cartilage ossification, and compared the morphologically and molecular biologically the SD rats and CCI rats. From the results of micro CT images, HE staining and SOFG staining, it was considered that the increase in cartilage width and delayed ossification leads to low growth. Based on the BMD value and real time PCR of DMP 1 and ISH results, it was considered that there is no difference between SD rats and CCI rats concerning bone density and bone maturation. BrdU immunostaining showed excessive proliferation of chondrocytes in CCI rats. In addition, since the expression distribution of Gli1 was widely recognized, it was suggested that chondrocyte maturation in growth cartilage. However, since there is no difference in the expression of Ihh. These findings suggest that the ossification of CCI rats is highly likely to be an abnormality in the signal system associated with independent Gli1, unlike ossification control by the Ihh signal system. The morphological abnormality of CCI rats was due to hyperproliferation of chondrocytes and immature maturation of hypertrophic chondrocytes, suggesting the possibility that expression and distribution abnormality of Gli1 and DMP1 may be involved in the onset mechanism. ","subitem_description_type":"Other"}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座歯科矯正学分野\nDepartment of Orthodontics, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"聖マリアンナ医科大学薬理学教室\nDepartment of Pharmacology, St. Marianna University School of Medicine"},{"subitem_textarea_value":"聖マリアンナ医科大学大学院実験動物飼育管理研究施設\nInstitute for Animal Experimentation, St. Marianna University Graduate School of Medicine"},{"subitem_textarea_value":"東京医科歯科大学大学院医歯学総合研究科口腔基礎工学分野\nDepartment of Basic Oral Health Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University"},{"subitem_textarea_value":"東京医科歯科大学大学院医歯学総合研究科顎顔面解剖学分野\nDepartment of Maxillofacial Anatomy, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座歯科矯正学分野\nDepartment of Orthodontics, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹内, 綾"}],"nameIdentifiers":[{"nameIdentifier":"3809","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000367835138","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000367835138"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"永山, 元彦","creatorNameLang":"ja"},{"creatorName":"NAGAYAMA, MOTOHIKO","creatorNameLang":"en"}],"familyNames":[{"familyName":"永山","familyNameLang":"ja"},{"familyName":"NAGAYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"元彦","givenNameLang":"ja"},{"givenName":"MOTOHIKO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1290","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050298436","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050298436"},{"nameIdentifier":"50298436","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50298436"}]},{"creatorNames":[{"creatorName":"田中, 政巳"}],"nameIdentifiers":[{"nameIdentifier":"15384","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000021215954","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000021215954"}]},{"creatorNames":[{"creatorName":"渡辺, 実"}],"nameIdentifiers":[{"nameIdentifier":"15385","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000000637361","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000637361"}]},{"creatorNames":[{"creatorName":"天野, 均"}],"nameIdentifiers":[{"nameIdentifier":"15386","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柴田, 俊一"}],"nameIdentifiers":[{"nameIdentifier":"15387","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014431415","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014431415"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"朝日大学歯学部口腔構造機能発育学講座歯科矯正学分野","affiliationNameLang":"ja"},{"affiliationName":"Department of Orthodontics, Division of Oral Structure, Function and Development, Asahi University School of Dentistry","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"北井, 則行","creatorNameLang":"ja"},{"creatorName":"KITAI, NORIYUKI","creatorNameLang":"en"}],"familyNames":[{"familyName":"北井","familyNameLang":"ja"},{"familyName":"KITAI","familyNameLang":"en"}],"givenNames":[{"givenName":"則行","givenNameLang":"ja"},{"givenName":"NORIYUKI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3902","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020271025","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000020271025"},{"nameIdentifier":"20271025","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20271025"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-30"}],"displaytype":"detail","filename":"gifushika461_2739_2019.pdf","filesize":[{"value":"16.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika461_2739_2019","url":"https://asahi-u.repo.nii.ac.jp/record/12185/files/gifushika461_2739_2019.pdf"},"version_id":"6dfb473a-00d4-4f20-9e03-424c6470419d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"軟骨石灰化不全ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"CCIラット","subitem_subject_scheme":"Other"},{"subitem_subject":"頭蓋底軟骨結合","subitem_subject_scheme":"Other"},{"subitem_subject":"CCI Rat","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cranial base synchondrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"軟骨石灰化不全ラットにおける頭蓋底軟骨結合の形態学的ならびに分子生物学的検索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"軟骨石灰化不全ラットにおける頭蓋底軟骨結合の形態学的ならびに分子生物学的検索"},{"subitem_title":"Morphological and Molecular biology Study of Cranial Base Synchondrosis in Cartilage Calcification Insufficient Rat","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["446"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-23"},"publish_date":"2020-03-23","publish_status":"0","recid":"12185","relation_version_is_last":true,"title":["軟骨石灰化不全ラットにおける頭蓋底軟骨結合の形態学的ならびに分子生物学的検索"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-01-17T07:05:59.722843+00:00"}