{"created":"2023-06-23T12:20:45.953829+00:00","id":12193,"links":{},"metadata":{"_buckets":{"deposit":"bcb1d1f6-d737-4648-aaee-64bcd22404a5"},"_deposit":{"created_by":21,"id":"12193","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"12193"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00012193","sets":["46:348:447"]},"author_link":["1779","1337"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"85","bibliographicPageStart":"73","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"蛋白質の多くは、「第3の生命鎖」と呼ばれる糖鎖による翻訳後修飾を受けて、糖蛋白質として機能している。これまでの長い間、糖蛋白質の存在は真核生物のみに限られ、原核生物である細菌にはありえないと信じられてきた。ところが、ようやく近年になって、細菌においても糖蛋白質が発見されてきた。当初はCampylobacter属やNeisseria属で研究が始められ、そしてBacteroides属やその他の属にも拡大しつつある。この総説では、口腔細菌における糖蛋白質研究の状況を、代表的な歯周病関連細菌であるPorphyromonas gingivalisおよびTannerella forsythiaを中心にして紹介する。まず、P. gingivalisおよびT. forsythiaにおいて、これまでに報告された糖蛋白質を整理して示す。P. gingivalisでは、主としてC-terminal domain配列をもつ蛋白質が、IX型分泌機構によって、菌体の外側へと輸送され、その後にAnionic lipopolysaccharideの糖鎖で修飾される。T. forsythiaでも同様に、C-terminal domain配列をもつ蛋白質は、IX型分泌機構によって、菌体の外側へと輸送される。しかし、その後はGeneral O-glycosylation systemによって糖鎖修飾される点が大きく異なる。次に、著者らが行ってきた歯周病関連細菌における新たな糖蛋白質の検索の試みについて示す。その結果として、OmpA様蛋白質を含む数種類の新奇の糖蛋白質を見い出すことができた。さらに、データベース等を利用した糖鎖修飾に関わる酵素の推定についても合わせて述べることにする。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Many proteins are glycosylated with glycans, tagged the “third life chains”, during post-translational modification. It was long believed that glycoproteins were exclusively found in eukaryotes. In recent years, however, glycoproteins have been identified in bacteria such as Campylobacter and Neisseria, and studies on glycoproteins are gradually expanding to include Bacteroides and other genera. The focus of this review is the recent advances in glycoprotein studies in oral bacteria, especially representative periodontal pathogens, Porphyromonas gingivalis and Tannerella forsythia. First, the glycoproteins that have been characterized in P. gingivalis and T. forsythia are summarized. In P. gingivalis, proteins that possess a C-terminal domain are secreted outs of bacteria via the type IX secretion system. The secreted proteins are mostly glycosylated by binding to anionic lipopolysaccharide. In contrast, in T. forsythia, proteins with a C-terminal domain are exported by the same type IX secretion system ; however, exported proteins are glycosylated by their general O-glycosylation system. Next, the approaches for detection of novel glycoproteins in periodontal pathogens are presented, which helped identify several glycoproteins, including OmpA-like proteins. Lastly, using bioinformatic analysis, the glycoenzymes related to glycosylation of OmpA-like proteins are proposed. ","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"1779","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000258692374","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000258692374"}],"names":[{"name":"Murakami, Yukitaka"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"総説","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"REVIEW ARTICLE","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔科学共同研究所\nCentral Research Laboratories Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"33703","affiliationNameIdentifierScheme":"kakenhi","affiliationNameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=33703"}],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"村上, 幸孝","creatorNameLang":"ja"},{"creatorName":"MURAKAMI, Yukitaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"村上","familyNameLang":"ja"},{"familyName":"MURAKAMI","familyNameLang":"en"}],"givenNames":[{"givenName":"幸孝","givenNameLang":"ja"},{"givenName":"Yukitaka","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1337","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000258692374","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000258692374"},{"nameIdentifier":"60239506","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60239506"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-30"}],"displaytype":"detail","filename":"gifushika462_7385_2019.pdf","filesize":[{"value":"597.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika462_7385_2019","url":"https://asahi-u.repo.nii.ac.jp/record/12193/files/gifushika462_7385_2019.pdf"},"version_id":"ea3a928e-621d-430c-854c-15d73319837d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯周病関連細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"糖蛋白質","subitem_subject_scheme":"Other"},{"subitem_subject":"糖鎖修飾","subitem_subject_scheme":"Other"},{"subitem_subject":"糖鎖修飾酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal bacteria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycoprotein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycosylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycoenzyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"歯周病関連細菌の糖蛋白質と糖鎖修飾に関わる酵素","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"歯周病関連細菌の糖蛋白質と糖鎖修飾に関わる酵素"},{"subitem_title":"Glycoproteins and Glycoenzymes in Periodontal Bacteria","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["447"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-25"},"publish_date":"2020-03-25","publish_status":"0","recid":"12193","relation_version_is_last":true,"title":["歯周病関連細菌の糖蛋白質と糖鎖修飾に関わる酵素"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-04-30T09:29:12.270307+00:00"}