{"created":"2023-06-23T12:21:06.593448+00:00","id":12619,"links":{},"metadata":{"_buckets":{"deposit":"f2aa72dd-a66f-4067-99b9-18a966310d0a"},"_deposit":{"created_by":21,"id":"12619","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"12619"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00012619","sets":["46:348:456"]},"author_link":["3930","3933","14447","3934","3931","14448","15756","15755"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"28","bibliographicPageStart":"21","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"mecAはβ-ラクタム系抗菌薬が結合できないペプチドグリカン合成酵素PBP2’ をコードする遺伝子であり、メチシリン耐性黄色ブドウ球菌（MRSA）はこの遺伝子を獲得することでβ-ラクタム耐性を獲得している。mecA遺伝子は可動性染色体カセット（SCCmec）を構成する一遺伝子として水平的に伝播し、黄色ブドウ球菌以外のブドウ球菌（コアグラーゼ陰性ブドウ球菌、CoNS）もSCCmecの獲得によってβ-ラクタム耐性を獲得しうる。SCCmecは構成や配列の異なる複数のタイプが存在するため、その解析によってmecA遺伝子の伝播状況が推測できる。本研究では、本学学生143名を被検者としてmecA陽性ブドウ球菌の検出を行い、そのSCCmecのタイプを解析することでmecA遺伝子の伝播状況の推測を行うことを目的とした。被検者から鼻腔検体を採取し、β-ラクタム系抗菌薬セフォキシチンと高濃度食塩を含む選択鑑別分離培地で培養して検出されたブドウ球菌について、mecA遺伝子の有無とそのSCCmecのタイプをPCR法で解析した。その結果、143名中65名（45%）から合計86菌株のmecA陽性ブドウ球菌が検出された。さらに、mecA陽性ブドウ球菌86株中84株（98%）は黄色ブドウ球菌ではなく、CoNSであることが分かった。これらの菌株は市中でも頻繁にみられるSCCmec type Ⅳ aを保有していた。これらの結果から、ヒトの鼻腔や皮膚の常在菌であるCoNSが、他のブドウ球菌にmecA遺伝子を水平的に伝播しながらmecA陽性ブドウ球菌が拡散している可能性が示唆された。今後は、常在菌が潜在的な薬剤耐性菌となりうる危険性を考慮して、薬剤耐性に対するアクションプランを推進していかなければならないと考えられる。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The mecA gene encodes for peptidoglycan synthase PBP2’ protein that escapes binding of β-lactam antibiotics, by which methicillin-resistant Staphylococcus aureus (MRSA) acquires antibiotic resistance. mecA is known to be horizontally transferred by mobile chromosome cassette (SCCmec) which acts as a vehicle that enables acquisition of β-lactam resistance of staphylococcal species, including Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Since SCCmec elements are classified into many types, investigation of the SCCmec types in isolated mecA-positive staphylococci might make the prediction of propagation of mecA among commensal staphylococci possible. The purpose of this study was to detect mecA-positive staphylococci from a random population of 143 students of our university as subjects, and to analyze SCCmec types to estimate the propagation of mecA. Nasal swab specimens were collected from the subjects and were cultured in a selective agar media containing the β-lactam antibiotic cefoxitin and a high concentration of sodium chloride. The presence or absence of mecA and the type of SCCmec for those β-lactam-resistant staphylococci were analyzed by PCR. As a result, a total of 86 strains of mecA-positive staphylococci were detected in 65 out of 143 subjects studied (45%). Furthermore, analysis of the detected mecA-positive staphylococci revealed that 84 of the 86 strains (98%) were belonging to the CoNS type, but not S. aureus. These strains possessed SCCmec type IVa, which is frequently found in community-acquired MRSA. Hence, the results suggest that CoNS, a resident staphylococcal type present in the human nasal cavity and skin, might transfer mecA gene to other staphylococci in the nasal cavity, resulting in the spread of mecA-positive staphylococci. In the future, it is necessary to promote an action plan for drug resistance in consideration of the danger of commensal bacteria potentially becoming drug-resistant bacteria. ","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"15755","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KATAOKA, HIDEO"}]},{"nameIdentifiers":[{"nameIdentifier":"15756","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MORI, TAIKI"}]},{"nameIdentifiers":[{"nameIdentifier":"3933","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOMATA, MEGUMI"}]},{"nameIdentifiers":[{"nameIdentifier":"3934","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INTO, TAKESHI"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座口腔微生物学分野\nDepartment of Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座口腔微生物学分野\nDepartment of Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座口腔微生物学分野\nDepartment of Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座口腔微生物学分野\nDepartment of Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"片岡, 嗣雄"}],"nameIdentifiers":[{"nameIdentifier":"14447","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004727966","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004727966"}]},{"creatorNames":[{"creatorName":"森, 大気"}],"nameIdentifiers":[{"nameIdentifier":"14448","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"猪俣, 恵"}],"nameIdentifiers":[{"nameIdentifier":"3930","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000017728337","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000017728337"},{"nameIdentifier":"40553798","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40553798"}]},{"creatorNames":[{"creatorName":"引頭, 毅"}],"nameIdentifiers":[{"nameIdentifier":"3931","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004727967","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004727967"},{"nameIdentifier":"10360918","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10360918"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-08-26"}],"displaytype":"detail","filename":"gifushika471_2128_2020.pdf","filesize":[{"value":"782.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika471_2128_2020","url":"https://asahi-u.repo.nii.ac.jp/record/12619/files/gifushika471_2128_2020.pdf"},"version_id":"ee2d3cba-6648-4518-b6ab-06651fdfc2ba"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mecA","subitem_subject_scheme":"Other"},{"subitem_subject":"メチシリン耐性コアグラーゼ陰性ブドウ球菌","subitem_subject_scheme":"Other"},{"subitem_subject":"MR-CoNS","subitem_subject_scheme":"Other"},{"subitem_subject":"常在菌","subitem_subject_scheme":"Other"},{"subitem_subject":"mecA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"methicillin-resistant coagulase-negative staphylococci","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MR-CoNS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"resident flora","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"本学学生から検出されたmecA陽性ブドウ球菌の解析とmecA遺伝子伝播状況の推測","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"本学学生から検出されたmecA陽性ブドウ球菌の解析とmecA遺伝子伝播状況の推測"},{"subitem_title":"Analysis of mecA-positive staphylococci detected from the students of Asahi University and inferences of propagation of mecA gene among commensal staphylococci","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["456"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-26"},"publish_date":"2020-08-26","publish_status":"0","recid":"12619","relation_version_is_last":true,"title":["本学学生から検出されたmecA陽性ブドウ球菌の解析とmecA遺伝子伝播状況の推測"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-09-15T06:03:46.631600+00:00"}