{"created":"2023-06-23T12:13:15.652121+00:00","id":1273,"links":{},"metadata":{"_buckets":{"deposit":"c04eadab-bcea-41e2-886b-9128a66d29a0"},"_deposit":{"created_by":20,"id":"1273","owners":[20],"pid":{"revision_id":0,"type":"depid","value":"1273"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00001273","sets":["49:132:367"]},"author_link":["1784","1780","1786","1788","1782","1787","1783","1779","1781","1785"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"493","bibliographicPageStart":"485","bibliographicVolumeNumber":"87","bibliographic_titles":[{},{"bibliographic_title":"Biotechnic & histochemistry : official publication of the Biological Stain Commission","bibliographic_titleLang":"en"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane. We examined the relationship between high molecular weight proteins and the S-layer using electron microscopic immunolabeling with chemical fixation and an antigen retrieval procedure consisting of heating in a microwave oven or autoclave with citraconic anhydride. Immunogold particles were localized clearly at the outermost cell surface. We also used energy-filtering transmission electron microscopy (EFTEM) to visualize 3, 3'-diaminobenzidine tetrahydrochloride (DAB) reaction products after microwave antigen retrieval with 1% citraconic anhydride. The three-window method for electron spectroscopic images (ESI) of nitrogen by the EFTEM reflected the presence of moieties demonstrated by the DAB reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies instead of immunogold particles. The mapping patterns of net nitrogen were restricted to the outermost cell surface.","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane. We examined the relationship between high molecular weight proteins and the S-layer using electron microscopic immunolabeling with chemical fixation and an antigen retrieval procedure consisting of heating in a microwave oven or autoclave with citraconic anhydride. Immunogold particles were localized clearly at the outermost cell surface. We also used energy-filtering transmission electron microscopy (EFTEM) to visualize 3, 3'-diaminobenzidine tetrahydrochloride (DAB) reaction products after microwave antigen retrieval with 1% citraconic anhydride. The three-window method for electron spectroscopic images (ESI) of nitrogen by the EFTEM reflected the presence of moieties demonstrated by the DAB reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies instead of immunogold particles. The mapping patterns of net nitrogen were restricted to the outermost cell surface.","subitem_description_type":"Other"}]},"item_10001_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"22984898","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.3109/10520295.2012.687117","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"出版社版"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://informahealthcare.com/doi/pdf/10.3109/10520295.2012.687117","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1473-7760","subitem_source_identifier_type":"ISSN"}]},"item_10001_textarea_24":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"Oral Anatomy, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Oral Pathology, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Endodontics, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Microbiology, School of Dentistry, Aichi-Gakuin University"},{"subitem_textarea_value":"Endodontics, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"Oral Pathology, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Microbiology, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Endodontics, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University"},{"subitem_textarea_value":"Oral Anatomy, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University"}]},"item_10001_textarea_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_textarea_value":"Informa healthcare"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Moriguchi, K"}],"nameIdentifiers":[{"nameIdentifier":"1780","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitamura, Y"}],"nameIdentifiers":[{"nameIdentifier":"1781","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwami, J"}],"nameIdentifiers":[{"nameIdentifier":"1782","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hasegawa, Y"}],"nameIdentifiers":[{"nameIdentifier":"1783","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, N"}],"nameIdentifiers":[{"nameIdentifier":"1784","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murakami, Yukitaka"}],"nameIdentifiers":[{"nameIdentifier":"1779","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000258692374","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000258692374"}]},{"creatorNames":[{"creatorName":"Maeda, H"}],"nameIdentifiers":[{"nameIdentifier":"1785","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshimura, F"}],"nameIdentifiers":[{"nameIdentifier":"1786","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, H"}],"nameIdentifiers":[{"nameIdentifier":"1787","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohno, N"}],"nameIdentifiers":[{"nameIdentifier":"1788","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-01-08"}],"displaytype":"detail","filename":"10520295_87_485493_2012.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"10520295_87_485493_2012","url":"https://asahi-u.repo.nii.ac.jp/record/1273/files/10520295_87_485493_2012.pdf"},"version_id":"8ff9d1f9-8c27-4a90-9d7d-ec251846b20d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"3,3'-Diaminobenzidine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Antigens, Bacterial","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacterial Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacteroides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Citraconic Anhydrides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Heating","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Horseradish Peroxidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Membrane Glycoproteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microscopy, Energy-Filtering Transmission Electron","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microwaves","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride."},{"subitem_title":"Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride.","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"20","path":["367"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-01-08"},"publish_date":"2015-01-08","publish_status":"0","recid":"1273","relation_version_is_last":true,"title":["nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride."],"weko_creator_id":"20","weko_shared_id":20},"updated":"2023-06-23T13:49:26.149540+00:00"}