{"created":"2023-06-23T12:21:51.876070+00:00","id":13586,"links":{},"metadata":{"_buckets":{"deposit":"f85e016b-7602-4d4d-a50f-1690b43cca03"},"_deposit":{"created_by":21,"id":"13586","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"13586"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00013586","sets":["46:348:481"]},"author_link":["3998","4042","15764","3561","1290","12706","16620","2875"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"125","bibliographicPageStart":"115","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"口腔粘膜の細胞診は従来ブラシ等で粘膜の病巣を擦過し、そのままスライドグラスに塗抹し、その後、アルコール等で固定あるいは風乾する方法、いわゆる従来法で行われていた。この従来法では1回の検体収量が少なく、基本的なPapanicolaou染色（PAP染色）とMay-Giemsa染色の2種類程度の検索しか行うことができなかった。また、細胞がスライドグラスの一部に密集してしまうことも多く、塗抹後の標本の乾燥や固定不足等の問題も回避できないものであった。これらの、従来法細胞診における問題点を解決するために、近年、採取に用いたブラシ等から直接専用の固定液に細胞を浮遊させ固定し、その後、専用の装置を用いスライドグラスに均一に塗抹する液状化検体細胞診（liquid-based cytology、LBC）が導入されつつある。これまで坂野らは4-nitroquinoline 1-oxide（4NQO）を用いたラット舌発がん実験により、heterogeneous nuclear ribonucleic protein K（hnRNP K）の検索を行ってきた。hnRNP Kは、ラット第\n17番染色体に含まれるがん関連遺伝子の1つであるRNA結合タンパクhnRNPファミリーの1 つで、主にmRNAと結合してmRNAの核外輸送、転写、翻訳および修飾に関与している。ラット舌がんにおける正常の粘膜組織と比較したmRNA発現検討でhnRNP KmRNAはTP53mRNAに比較して上皮性異形成において有意にタンパクの高発現がみられ、hnRNP Kは前がん病変の診断マーカーとして組織学的に有用であると報告されている。今回、ラット舌発がんモデルを用いて細胞診と組織診を比較し診断の精度について検討し、さらにhnRNP Kとp53の免疫組織化学的染色や、mRNA発現量比較検討への応用による診断精度向上の可能性についても検討した。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Oral cytology has been performed mainly by using the conventional method, in which the lesion of the mucosa is scraped, e.g., with a brush, and then the collected cells are smeared on a slide glass. Subsequently, the slide is fixed with alcohol or air dry. However, in this method, a limited amount of cells can be obtained at one time, and only Papanicolaou and May-Giemsa staining can be used for examination. Also, obtained cells are more likely to overlap on the slide glass, and other inevitable problems include drying of the specimen and insufficient fixation. Liquid-based cytology (LBC) can be performed without these disadvantages ; therefore, it has become widely used recently. In this method, cells are collected, e.g., with a brush, and it is placed directly into a fixing solution. Then the cells can be evenly smeared on the slide glass in a laboratory. Sakano et al. have searched for heterogeneous nuclear ribonucleic protein K (hnRNP K) in a 4-nitroquinoline 1-oxide (4NQO) -induced rat tongue carcinogenesis. hnRNP K is a member of the hnRNP family, an RNA-binding protein of a cancer-related genes located on rat chromosome 17. This protein mainly binds to mRNA and is involved in nuclear transport, transcription, translation and modification of mRNA.4, 5) In a study of mRNA expression between normal mucosa and cancer lesion in rat carcinogenesis model, in epithelial dysplasia, protein expression of hnRNP K mRNA was significantly higher than that of TP53 and hnRNP K, indicating that hnRNP K was histologically useful as a diagnostic marker for precancerous lesions. Here, we investigated diagnostic accuracy between cytology and histology in rat carcinogenesis models and the possibility of the improvement of diagnostic accuracy of LBC by using immunohistochemistry of hnRNP K and p53 and analysis of mRNA expression level of these genes.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"15764","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KANEKO, HIROYASU"}]},{"nameIdentifiers":[{"nameIdentifier":"16620","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NAKAO, JUNA"}]},{"nameIdentifiers":[{"nameIdentifier":"4042","nameIdentifierScheme":"WEKO"}],"names":[{"name":"EHARA, MICHIKO"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"朝日大学歯学部","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"永山","familyNameLang":"ja"},{"familyName":"NAGAYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"元彦","givenNameLang":"ja"},{"givenName":"MOTOHIKO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1290","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050298436","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050298436"},{"nameIdentifier":"50298436","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50298436"}],"names":[{"name":"永山, 元彦","nameLang":"ja"},{"name":"NAGAYAMA, MOTOHIKO","nameLang":"en"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"住友","familyNameLang":"ja"},{"familyName":"SUMITOMO","familyNameLang":"en"}],"givenNames":[{"givenName":"伸一郎","givenNameLang":"ja"},{"givenName":"SHINICHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3998","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050216496","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050216496"},{"nameIdentifier":"50216496","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50216496"}],"names":[{"name":"住友, 伸一郎","nameLang":"ja"},{"name":"SUMITOMO, SHINICHIRO","nameLang":"en"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔外科学分野\nDepartment of Oral & Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔外科学分野\nDepartment of Oral & Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金子, 裕康"}],"nameIdentifiers":[{"nameIdentifier":"12706","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中尾, 寿奈"}],"nameIdentifiers":[{"nameIdentifier":"2875","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000387366480","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000387366480"}]},{"creatorNames":[{"creatorName":"江原, 道子"}],"nameIdentifiers":[{"nameIdentifier":"3561","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000251869792","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000251869792"},{"nameIdentifier":"10425308","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10425308"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"永山, 元彦","creatorNameLang":"ja"},{"creatorName":"NAGAYAMA, MOTOHIKO","creatorNameLang":"en"}],"familyNames":[{"familyName":"永山","familyNameLang":"ja"},{"familyName":"NAGAYAMA","familyNameLang":"en"}],"givenNames":[{"givenName":"元彦","givenNameLang":"ja"},{"givenName":"MOTOHIKO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1290","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050298436","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050298436"},{"nameIdentifier":"50298436","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50298436"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"住友, 伸一郎","creatorNameLang":"ja"},{"creatorName":"SUMITOMO, SHINICHIRO","creatorNameLang":"en"}],"familyNames":[{"familyName":"住友","familyNameLang":"ja"},{"familyName":"SUMITOMO","familyNameLang":"en"}],"givenNames":[{"givenName":"伸一郎","givenNameLang":"ja"},{"givenName":"SHINICHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3998","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050216496","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050216496"},{"nameIdentifier":"50216496","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50216496"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-29"}],"displaytype":"detail","filename":"gifushika473_115125_2021.pdf","filesize":[{"value":"39.6 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K","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p53","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"液状化検体細胞診を用いたラット舌発がん実験における診断への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"液状化検体細胞診を用いたラット舌発がん実験における診断への応用"},{"subitem_title":"Application of liquid-based cytology in diagnosis of oral precancerous lesion.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["481"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-29"},"publish_date":"2021-09-29","publish_status":"0","recid":"13586","relation_version_is_last":true,"title":["液状化検体細胞診を用いたラット舌発がん実験における診断への応用"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-01-17T07:09:11.527691+00:00"}