@article{oai:asahi-u.repo.nii.ac.jp:00013903,
 author = {小萱, 康徳 and 岩久, 文彦},
 issue = {2003-10-20},
 journal = {2003-10-20},
 month = {2003-10-20, 2022-05-17},
 note = {It is difficult to slam freeze odontoblasts that are surrounded by enamel organ and dental papilla, because they must first be mechanically exposed using a razor blade, which damage the cells. Furthermore, because the area optimally cryo-preserved is less than 10 um below the freezing surface, it is difficult to obtain specimens without mechanical or ice crystal induced damages. In this study, high-pressure freezing which permits cryo-fixation of tissues up to 0.5mm thick, was used to cryo-preserve rat odontoblasts. This method permits optimal cryo-preservation without ice and mechanical cell damage to the entire odontoblast layer. This method produced marked qualitative differences with regard to the mitochondria and various membrane-bounded structures.},
 pages = {2003-10-20--2003-10-20},
 title = {高圧急速凍結・凍結置換固定したラット象牙芽細胞},
 volume = {2003-10-20},
 year = {}
}