{"created":"2023-06-23T12:22:06.264081+00:00","id":13903,"links":{},"metadata":{"_buckets":{"deposit":"ceaa8978-8de6-4188-9611-74d48da7e349"},"_deposit":{"created_by":21,"id":"13903","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"13903"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00013903","sets":["46:190:421"]},"author_link":["3873","3866","4508","8752"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-10-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"147","bibliographicPageStart":"140","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is difficult to slam freeze odontoblasts that are surrounded by enamel organ and dental papilla, because they must first be mechanically exposed using a razor blade, which damage the cells. Furthermore, because the area optimally cryo-preserved is less than 10 um below the freezing surface, it is difficult to obtain specimens without mechanical or ice crystal induced damages. In this study, high-pressure freezing which permits cryo-fixation of tissues up to 0.5mm thick, was used to cryo-preserve rat odontoblasts. This method permits optimal cryo-preservation without ice and mechanical cell damage to the entire odontoblast layer. This method produced marked qualitative differences with regard to the mitochondria and various membrane-bounded structures.","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"エナメル器と歯乳頭に囲まれている象牙芽細胞を圧着法で凍結固定するためには,カミソリ刃を用いて細胞を露出しなければならない.この操作で象牙芽細胞は機械的な損傷を受けることになる.さらに適正に凍結固定される領域は圧着面から10μm以下であるので,機械的および氷結晶による損傷のない試料を得ることは極めて困難である.本研究では,0.5mm厚の組織の凍結固定が可能な高圧急速凍結法を用いてラット象牙芽細胞の微細構造を検索した.象牙芽細胞層全域で氷結晶や機械的損傷の無い凍結固定像が得られ,殊にミトコンドリアや膜周囲性構造物の微細構造に質的な違いが認められた.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"3866","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002269921","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002269921"},{"nameIdentifier":"30076046","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30076046"}],"names":[{"name":"KOGAYA, YASUTOKU"}]},{"nameIdentifiers":[{"nameIdentifier":"4508","nameIdentifierScheme":"WEKO"}],"names":[{"name":"IWAKU, FUMIHIKO"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110004689607","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_86":{"attribute_name":"医中誌文献番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2004180155","subitem_relation_type_select":"ICHUSHI"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔解剖学分野\nDepartment of Oral Anatomy, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔解剖学分野\nDepartment of Oral Anatomy, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小萱, 康徳"}],"nameIdentifiers":[{"nameIdentifier":"3873","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002269921","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002269921"},{"nameIdentifier":"30076046","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30076046"}]},{"creatorNames":[{"creatorName":"岩久, 文彦"}],"nameIdentifiers":[{"nameIdentifier":"8752","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000002269924","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000002269924"},{"nameIdentifier":"60018421","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60018421"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-17"}],"displaytype":"detail","filename":"gifushika302_140147_2003.pdf","filesize":[{"value":"4.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika302_140147_2003","url":"https://asahi-u.repo.nii.ac.jp/record/13903/files/gifushika302_140147_2003.pdf"},"version_id":"c937b1c0-3bb6-4a92-9b4c-e2a8e0c02833"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"高圧急速凍結","subitem_subject_scheme":"Other"},{"subitem_subject":"象牙芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア","subitem_subject_scheme":"Other"},{"subitem_subject":"検体取り扱い法","subitem_subject_scheme":"Other"},{"subitem_subject":"象牙芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア","subitem_subject_scheme":"Other"},{"subitem_subject":"凍結置換","subitem_subject_scheme":"Other"},{"subitem_subject":"超微構造","subitem_subject_scheme":"Other"},{"subitem_subject":"High-pressure freezing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"odontoblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondria","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"高圧急速凍結・凍結置換固定したラット象牙芽細胞","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高圧急速凍結・凍結置換固定したラット象牙芽細胞"},{"subitem_title":"Rat Odontoblasts as Revealed by High-Pressure Freezing Followed by Freeze-Substitution","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["421"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-17"},"publish_date":"2022-05-17","publish_status":"0","recid":"13903","relation_version_is_last":true,"title":["高圧急速凍結・凍結置換固定したラット象牙芽細胞"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-06-23T12:40:47.744560+00:00"}