{"created":"2023-06-23T12:22:12.816733+00:00","id":14031,"links":{},"metadata":{"_buckets":{"deposit":"907340f6-11a4-443f-a845-3413ee64316e"},"_deposit":{"created_by":21,"id":"14031","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"14031"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00014031","sets":["46:190:430"]},"author_link":["4093","1251","6391","6598","6548","4274"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"216","bibliographicPageStart":"207","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"歯肉の結節性過形成（エプーリス;GH）の形成機序を明らかにするために、数種の代表的成長因子とその特異的レセプターを免疫組織化学的に検出した。その検出に際して、GHは光顕的に、肉芽組織優位型（granulation dominent type;GDT）（病巣の60%以上が肉芽組織）（20例）、線維組織優位型（fibrous tissue dominant type;FDT）（60%以上が線維組織）（21例）そしてGDTやFDTに骨やセメント質が存在する骨・セメント形成型（cemento-ossifying type;COT）（21例）の3型に大別した。さらに、これらGHを細分類し、GDTはほとんど肉芽組織のみから成るType I（13例）、肉芽組織中に線維組織が疎らに形成されたType II（7例）に分け、FDTは40%以下の肉芽組織が混じるType III（7例）、肉芽組織がわずかで70-80%が線維組織より成るType IV（6例）、線維組織のみから成るType V（8例）に分けた。COTはType IIからType V中に硬組織を認め、GDTのType Iにはこれを認めなかった。免疫組織化学的検索の1次抗体は、増殖因子はepidermal growth factor(EGF),interleukin-1α(IL-1α),transforming growth factor-αおよび-β1(TGF-α,TGF-β1),platelet-derived growth factor(PDGF),basic fibroblast growth factor(b-FGF),tumor necrosis factor-αおよび-β(TNF-α,TNF-β),hepatocyte growth factor(HGF)を抗原としたものであり、これらに対するレセプターはEGFR,IL-1αR,TGF-βRI・TGF-βR II,PDGFR-α,Bek(FGFR II),TNFR II,HGFRに対する抗体とした。また、これらに加えて、マクロファージ(Mφ)を検出するためにCD68、トロポコラーゲンと線維芽細胞を検出するためにfibrobrast5B5(F5B5)抗体をも採用した。EGFはEGFRとともにいずれのエプーリスにも上皮以外は陰性、TNF-α,TNF-β1とTNFR II,HGFとHGFRはすべての症例においてすべての細胞が陰性であった。IL-αとIL-1αR,PDGFとPDGFR-α,b-FGFとBekはほぼすべての病型に頻度高く検出された。また、TGF-β1はV型を除くすべての病型の多くの細胞に陽性で、そのレセプターのTGF-βR Iは陰性か弱陽性を示し、TGF-βR IIはV型を除く各病型の線維芽細胞や血管芽細胞および骨・セメント質形成性エプーリスの間葉細胞に陽性であった。これらの増殖因子とそのレセプターに対する陽性反応はMφ、線維芽細胞、血管芽細胞、血管内皮細胞、そしてCOTに特異的な間葉系組織細胞に認められた。CD68を用いた検索では、Mφの浸潤や局在はGHの肉芽組織の増殖の程度に相関し、従って、CD68陽性細胞はType IやType IIに多く、Type Vでは極端に少ないことが判明した。F5B5はType I（6例）、TypeII（3例）、Type III（3例）とCOTのType II（3例）、Type IV（4例）にも検出された。これらの所見から、エプーリスにおける肉芽組織中の細胞の増殖のための主な成長因子とレセプターはIL-1α,b-FGF,TGF-β1とこれらの特異的レセプターであること、その主要な産生細胞はMφであり、パラクリン的に線維芽細胞や血管芽細胞が増殖することが示唆された。また、TGF-β1はIV型の線維芽細胞と線維細胞に最もよく発現したところから、線維芽細胞の増殖とともに線維細胞への成熟と膠原線維の形成に関わっている可能性と骨・セメント質形成性エプーリスの間葉系組織への分化にも関与していることが示唆された。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Immunohistochemical localization of some typical growth factors and their specific receptors in gingival hyperplasia, so-called epulis (GH), was examined in order to clarify the mechanism of GH formation. Prior to immunohistochemistry, GH was microsopically divided into granulation tissue-dominant types (GDT), consisting of more than 60% granulation tissue of GH (20 cases), fibrous-tissue dominant types, consisting of more than 60% fibrous tissue (FDT X21 cases), and cemento-ossifying-types, consisting of cemento and/or osseous tissue in GDT or FDT (COT) (22 cases). These three types of GH were further classified into small groups. GDT was classified into Type I, which was simplified by only one component of proliferation of granulation tissue (13 cases), and Type II, which was accompanied by sparsed formation of fibrous tissue (7cases). FDT was classified into Type III, consisting of fibrous tissue with less than 40% granulation tissue (7 cases), Type IV, consisting of more than 70-80% fibrous tissue and sparsely localized granulation tissue (6 cases), and Type V, consisting of only fibrous tissue (8 cases). COT showed the formation of hard tissues in Type II to Type V, but was investigated in Type I of GDT.\nAntibodies to some growth factors, epidermal growth factor (EGF), interleukin-1α (IL-1α), transforming growth factor-α (TGF-α), transforming growth factor- ẞ (TGF-ẞ ), platelet-derived growth factor ( PDGF), basic fibroblast growth factor (b-FGF ), tumor necrosis factor-α, and - ẞ ( TNF-α, - ẞ) and hepatocyte growth factor (HGF), and antibodies to their specific receptors, EGFR, IL-1αR, TGF- ẞ R 1, TGF- ẞ RII, PDGFR-α, Be (FGFR II), TNFR II, and HGFR, were used for the present immunohistochemistry. Adding to the above antibodies, antibodies to CD 68 and fibroblast 5B 5(F5B5) were also adopted to identify macrophage (Mф), and detect tropocollagen and/or fibroblast, respectively.\nEGF and EGFR were not recognized in tissue except the epithelium. The expression of TNF-α, TNF-ẞ1, TNFR II, HGF and HGFR were negative in all cells in all types of GH. In contrast to negative findings of such growth factors and receptors, IL-1α, IL-1αR, PDGF, PDGFR-α, b-FGF, and FGFR II were demonstrated in all types of GH. TGF- ẞ 1 and TGF- ẞ RII were positive in the GH except Type V. The growth factors and receptors were mainly found in the cytoplasms of Mф, fibroblasts, angioblasts, endothelial cells, and mesenchymal cells which differentiated and proliferated around the cemento-osseous tissue of COT.\nThe examination using antibody to CD 68 clarified the infiltration and/or localization of Mф corresponding to the degree of proliferation of granulation tissue of each type of GH. Thus, positive cells were abundant in Type I and Type II, while, they were rare in Type V.\nF5B5 was detected in some cases of Type I (6 cases), Type II(3 cases), and Type III (2cases). F5B5 was also\nfound in the 3 cases each of Type II and Type III, and 4cases of Type IV of COT\nThese findings on microscopy and immunohistochemistry indicate that the main growth factors and receptors leading proliferation of granulation tissue are IL-α, b -FGF, and TGF- ẞ 1, and their specific receptors. It was also to the identitfied that the main producer of these growth factors involved in granuloma formation is Mф, suggesting that the proliferation of fibroblasts and angioblasts is mediated by the paracrine system. The formation of collagen fibers which take a FDT direction was suggested to arise in Type I to Type III of GH with the formation of granulation tissue, and the mature fibers transform GH to epulis fibrosa. TGF- ẞ 1 and TGF- ẞ R II were suggested to participate in the transformation of GH to FDT in view of the function of TGF- ẞ 1.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"6598","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAWARADA, YUKO"}]},{"nameIdentifiers":[{"nameIdentifier":"6391","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SATO, MASARU"}]},{"nameIdentifiers":[{"nameIdentifier":"4274","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAKEUCHI, HIROSHI"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006607231","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_86":{"attribute_name":"医中誌文献番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2007210222","subitem_relation_type_select":"ICHUSHI"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔病理学分野\nDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川原田, 裕子"}],"nameIdentifiers":[{"nameIdentifier":"6548","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006648892","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006648892"}]},{"creatorNames":[{"creatorName":"佐藤, 勝"}],"nameIdentifiers":[{"nameIdentifier":"1251","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004584086","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004584086"},{"nameIdentifier":"70154101","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70154101"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"竹内, 宏","creatorNameLang":"ja"},{"creatorName":"TAKEUCHI, HIROSHI","creatorNameLang":"en"}],"familyNames":[{"familyName":"竹内","familyNameLang":"ja"},{"familyName":"TAKEUCHI","familyNameLang":"en"}],"givenNames":[{"givenName":"宏","givenNameLang":"ja"},{"givenName":"HIROSHI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4093","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040102123","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000040102123"},{"nameIdentifier":"40102123","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40102123"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-02-20"}],"displaytype":"detail","filename":"gifushika333_207216_2007.pdf","filesize":[{"value":"11.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika333_207216_2007","url":"https://asahi-u.repo.nii.ac.jp/record/14031/files/gifushika333_207216_2007.pdf"},"version_id":"bb840f41-a8e1-4cb3-b848-9d306fb9647f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"歯肉過形成","subitem_subject_scheme":"Other"},{"subitem_subject":"成長因子","subitem_subject_scheme":"Other"},{"subitem_subject":"レセプター","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫組織化学的局在","subitem_subject_scheme":"Other"},{"subitem_subject":"歯肉疾患","subitem_subject_scheme":"Other"},{"subitem_subject":"歯肉増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"肉芽組織","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫組織化学","subitem_subject_scheme":"Other"},{"subitem_subject":"gingival hyperplasia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"growth factors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"receptors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunohistochemical-localization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transforming Growth Factor Beta1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒト歯肉過形成(いわゆるエプーリス)における増殖因子とそのレセプターの免疫組織化学的局在","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト歯肉過形成(いわゆるエプーリス)における増殖因子とそのレセプターの免疫組織化学的局在"},{"subitem_title":"mmunohistochemical Localization of Some Growth Factors and Their Receptors in the Human Gingival Hyperplasia (so-called epulis)","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["430"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-20"},"publish_date":"2023-02-20","publish_status":"0","recid":"14031","relation_version_is_last":true,"title":["ヒト歯肉過形成(いわゆるエプーリス)における増殖因子とそのレセプターの免疫組織化学的局在"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-12-25T06:43:58.198520+00:00"}