{"created":"2023-06-23T12:22:13.581042+00:00","id":14046,"links":{},"metadata":{"_buckets":{"deposit":"041536e7-b3ef-4d1f-ae78-db27a7b6cde5"},"_deposit":{"created_by":21,"id":"14046","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"14046"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00014046","sets":["46:190:429"]},"author_link":["16854","16856","3134","16853","16855","4561","6425","4106","1598"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-10-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"120","bibliographicPageStart":"116","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Erk5は近年発見されたMAPKの新しいファミリー分子であるが、その機能はあまり明らかにされていない。本研究でわれわれは、胎生期顎下腺のErk5について検討を行った。ウエスタンブロット法による解析から、マウス顎下腺にはErk5が発現していることがわかった。また、胎仔マウス顎下腺に発現しているErk1/2とErk5がEGF（epidermal growth factor）刺激に応答性を示すのか否かについて調べた。胎生14日齢（E14）、胎生16日齢（E16）および胎生18日齢（E18）の顎下腺にEGF（50ng/ml）を添加して0分、5分、10分、30分および60分間培養した。刺激後の顎下腺を回収し、一定量のタンパク質（10μgあるいは50μg）をSDS-ポリアクリルアミドゲルで分離し、リン酸化Erk1/2抗体ならびにリン酸化Erk5抗体を用いてウエスタンブロット解析を行った。Erk1/2は全ての胎生齢でEGFの刺激に応答したリン酸化の上昇が認められた。しかし、Erk5のリン酸化はE14,E16で促進されたが、E18ではほとんど変化がないことがわかった。Erk1/2とErk5が顎下腺の分枝形態形成に及ぼす機能を調べるためにU0126を添加して48時間器官培養したところ、対照の顎下腺と比較して著しい分枝形成の抑制が認められた。以上の結果から、Erk5はErk1/2と同様に発達過程の顎下腺における分枝形成に重要な役割を持つものと考えられた。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Erk 5, also called \"big mitogen-activated protein kinase 1 (BMK1 )\", was recently found as a member of MAPK, and its biological roles are largely unknown. In this study, we investigated the expression of Erk 1/2 and Erk 5 in developing fetal mouse submandibular gland (SMG). Phosphorylation of Erk 1/2 was clearly induced by epidermal growth factor (EGF) of SMG rudiments at embryonic day 14 (E 14), embryonic day 16 ( E 16 ) and embryonic day 18( E 18); however, the phosphorylation of Erk 5 by EGF was observed at E 14 and E 16, but not at E 18. Branching morphogenesis of cultured embryonic day 13 ( E 13 ) SMG rudiments was suppressed by administration of a MEK specific inhibitor, U 0126, is known to inhibit the activation of both Erk 1/2 and Erk 5. Therefore, Erk 1/2 and Erk 5 cascade might play an important role in the regulation of branching morphogenesis of fetal mouse SMG.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"6425","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KOYAMA, NORIKO"}]},{"nameIdentifiers":[{"nameIdentifier":"16855","nameIdentifierScheme":"WEKO"}],"names":[{"name":"OHNO, KENJI"}]},{"nameIdentifiers":[{"nameIdentifier":"16856","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MATUMOTO, TUGUTERU"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"高井","familyNameLang":"ja"},{"familyName":"TAKAI","familyNameLang":"en"}],"givenNames":[{"givenName":"良招","givenNameLang":"ja"},{"givenName":"YOSHIAKI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4106","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070197044","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000070197044"},{"nameIdentifier":"70197044","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70197044"}],"names":[{"name":"高井, 良招","nameLang":"ja"},{"name":"TAKAI, YOSHIAKI","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"4561","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KASHIMATA, MASANORI"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006607211","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_86":{"attribute_name":"医中誌文献番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2007068106","subitem_relation_type_select":"ICHUSHI"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座高齢者歯科学分野\nDepartment of Geriatric Dentistry, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座高齢者歯科学分野\nDepartment of Geriatric Dentistry, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry"}]},"item_10002_textarea_78":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_textarea_value":"岐阜歯科学会"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小山, 典子"}],"nameIdentifiers":[{"nameIdentifier":"1598","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004728063","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004728063"}]},{"creatorNames":[{"creatorName":"大野, 健二"}],"nameIdentifiers":[{"nameIdentifier":"16853","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006297167","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006297167"}]},{"creatorNames":[{"creatorName":"松本, 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kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika332_116120_2006","url":"https://asahi-u.repo.nii.ac.jp/record/14046/files/gifushika332_116120_2006.pdf"},"version_id":"805b3513-6f81-497b-9fe6-97d4be7b54b0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Erk5","subitem_subject_scheme":"Other"},{"subitem_subject":"Erk1/2","subitem_subject_scheme":"Other"},{"subitem_subject":"胎仔マウス顎下腺","subitem_subject_scheme":"Other"},{"subitem_subject":"分枝形態形成","subitem_subject_scheme":"Other"},{"subitem_subject":"シグナル伝達","subitem_subject_scheme":"Other"},{"subitem_subject":"器官形成","subitem_subject_scheme":"Other"},{"subitem_subject":"ICRマウス","subitem_subject_scheme":"Other"},{"subitem_subject":"MAP Kinase 7","subitem_subject_scheme":"Other"},{"subitem_subject":"Erk5","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Erkl/2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fetal mouse SMG","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Branching morphogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Signal transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MAPK7","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"胎仔マウス顎下腺における新規MAPキナーゼErk5の発現と機能的役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎仔マウス顎下腺における新規MAPキナーゼErk5の発現と機能的役割"},{"subitem_title":"The Role of Erk5 in Developing Fetal Mouse Submandibular Gland","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["429"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-02-21"},"publish_date":"2023-02-21","publish_status":"0","recid":"14046","relation_version_is_last":true,"title":["胎仔マウス顎下腺における新規MAPキナーゼErk5の発現と機能的役割"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-12-25T07:37:19.604906+00:00"}