{"created":"2023-06-23T12:22:16.353252+00:00","id":14102,"links":{},"metadata":{"_buckets":{"deposit":"1de9f532-7c80-44fa-bee4-defcd013c902"},"_deposit":{"created_by":21,"id":"14102","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"14102"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00014102","sets":["46:190:427"]},"author_link":["1764","5367","8745","8796","6506","6533","5469","16863","8795","8604","1770","16861","6515","5465"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2-3","bibliographicPageEnd":"11","bibliographicPageStart":"7","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the effects of peroxynitrite on the activity of protein phosphatase 1 (PP 1), a metalloenzyme with putative important sulfhydryl groups for its activity. PP 1 activity was assayed colormetrically with a synthetic substrate of p-nitrophenyl phosphate. Peroxynitrite inactivated 16.7 nM PP 1 in a few seconds with an IC_50 =0.45 μM. The peroxynitrite decomposition products, nitrite and nitrate, had no effect on activity. Inactivation was restored by Mn^<2+> or dithiothreitol but not by Fe^<2+>  or sodium arsenite. These results show that peroxynitrite rapidly and reversibly inactivates PP 1 by affecting its sulfhydryl groups and Mn^<2+> .","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"細菌性毒素や炎症性サイトカイン等の刺激によりマクロファージなどで大量に産生される一酸化窒素（NO）は、感染や腫瘍に対し防御反応として作用する一方、宿主に対しても細胞・組織障害を引き起こすと考えられてきた。しかし、生体内ではNOそのものには従来言われているほどの高い化学反応性や毒性はなく、より反応性の高いペロキシナイトライト（ONOO-）のような二次酸化物が障害因子の本体ではないかと考えられるようになった。そこで、蛋白合成や神経伝達に重要な役割を持つ蛋白リン酸酵素1（protein phosphatase1:PP1）活性に及ぼすONOO-の作用を検討した。P-ニトロホスホフェノール（pNPP）がPP1によりニトロフェノールに変換される量を吸光度（410nm）により測定し、活性を評価した.ONOO-はPP1活性を用量依存性に抑制した（IC50=0.45μM）。ONOO-分解産物であるNO2やNO3にはPP1活性抑制作用はなかった。還元剤DTTにより抑制された活性は完全に回復したが、NaAsO2によっては回復しなかった。また、PP1活性の必須金属であるMn2+投与によって活性は回復した。酵素基質pNPP前処置はONOO-による抑制を一部のみ防御した。ONOO-は基質結合部位以外でジスルフィド結合させることによりMn2+の結合を減弱し、PP1活性を抑制する。これは、急性臓器不全の病因の一つである可能性を示唆している。","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"5367","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAKAKURA, KO"}]},{"nameIdentifiers":[{"nameIdentifier":"6533","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KURASHIGE, SYUHEI"}]},{"nameIdentifiers":[{"nameIdentifier":"6515","nameIdentifierScheme":"WEKO"}],"names":[{"name":"UENO, TAKAHIRO"}]},{"nameIdentifiers":[{"nameIdentifier":"16863","nameIdentifierScheme":"WEKO"}],"names":[{"name":"NIWA, HIKARU"}]},{"nameIdentifiers":[{"nameIdentifier":"8796","nameIdentifierScheme":"WEKO"}],"names":[{"name":"HIROSE, MASAYUKI"}]},{"nameIdentifiers":[{"nameIdentifier":"5469","nameIdentifierScheme":"WEKO"}],"names":[{"name":"OOSHIMA, KAZUYUKI"}]},{"nameIdentifiers":[{"nameIdentifier":"1764","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010231614","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010231614"}],"names":[{"name":"Mizogami, Maki"}]}]},"item_10002_heading_98":{"attribute_name":"見出し","attribute_value_mlt":[{"subitem_heading_banner_headline":"原著","subitem_heading_language":"ja"},{"subitem_heading_banner_headline":"ORIGINAL ARTICLES","subitem_heading_language":"en"}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110006155030","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_86":{"attribute_name":"医中誌文献番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2006235826","subitem_relation_type_select":"ICHUSHI"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"},{"subitem_textarea_value":"Department of Anesthesiology, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高倉, 康"}],"nameIdentifiers":[{"nameIdentifier":"8604","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006090746","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006090746"},{"nameIdentifier":"40206735","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40206735"}]},{"creatorNames":[{"creatorName":"倉茂, 秀平"}],"nameIdentifiers":[{"nameIdentifier":"8745","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006297137","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006297137"},{"nameIdentifier":"60584026","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60584026"}]},{"creatorNames":[{"creatorName":"上野, 高広"}],"nameIdentifiers":[{"nameIdentifier":"6506","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006297138","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006297138"}]},{"creatorNames":[{"creatorName":"丹羽, ひかる"}],"nameIdentifiers":[{"nameIdentifier":"16861","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006297139","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006297139"}]},{"creatorNames":[{"creatorName":"広瀬, 雅之"}],"nameIdentifiers":[{"nameIdentifier":"8795","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006297140","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006297140"}]},{"creatorNames":[{"creatorName":"大嶋, 和之"}],"nameIdentifiers":[{"nameIdentifier":"5465","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006090748","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006090748"}]},{"creatorNames":[{"creatorName":"溝上, 真樹"}],"nameIdentifiers":[{"nameIdentifier":"1770","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000010231614","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000010231614"},{"nameIdentifier":"10231614","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10231614"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-03-06"}],"displaytype":"detail","filename":"gifushika322.323_711_2006.pdf","filesize":[{"value":"446.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika322.323_711_2006","url":"https://asahi-u.repo.nii.ac.jp/record/14102/files/gifushika322.323_711_2006.pdf"},"version_id":"40967007-c6ae-48a4-80c2-a4a0f6a98a5e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ペロキシナイトライト","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白リン酸酵素1","subitem_subject_scheme":"Other"},{"subitem_subject":"急性臓器不全","subitem_subject_scheme":"Other"},{"subitem_subject":"歯科麻酔","subitem_subject_scheme":"Other"},{"subitem_subject":"一酸化窒素","subitem_subject_scheme":"Other"},{"subitem_subject":"Peroxynitrite","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein phosphatase 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"acute organ failure","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitrophenols","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phosphoric Monoester Hydrolases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Peroxynitrous Acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ペロキシナイトライトによる蛋白リン酸酵素1活性の抑制","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ペロキシナイトライトによる蛋白リン酸酵素1活性の抑制"},{"subitem_title":"Reversible Inhibition of Protein Phosphatasel by Peroxynitrite","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["427"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-03-06"},"publish_date":"2023-03-06","publish_status":"0","recid":"14102","relation_version_is_last":true,"title":["ペロキシナイトライトによる蛋白リン酸酵素1活性の抑制"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-06-23T12:39:25.540353+00:00"}