{"created":"2023-12-02T04:59:02.799858+00:00","id":2000077,"links":{},"metadata":{"_buckets":{"deposit":"739817ce-cde6-4b5d-965a-6287f0a9f8dd"},"_deposit":{"created_by":21,"id":"2000077","owner":"21","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"2000077"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:02000077","sets":["46:348:1694594900953"]},"author_link":["16902"],"control_number":"2000077","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicNumberOfPages":"9","bibliographicPageEnd":"48","bibliographicPageStart":"39","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"口腔擦過細胞診における器具および標本作製法別にみた細胞回収に影響する要素を、器具の観察と回収した細胞を定量的に比較し考察した。ボランティア5名を対象に、左舌縁を歯垢染色液で染め、オーセレックスブラシ®（OB）、歯間ブラシ（IB）および滅菌綿棒（CS）を用いて、擦過採取後の従来法と液状化検体細胞診（LBC）による標本作製過程の器具表面の観察および各器具で採取後の総採取量（TG）、ガラス表面やLBC保存液中の回収量（R1）をゲノムDNA量に見立て統計学的に比較した。細胞の採取状態は各器具で異なったが、いずれの器具・標本作製法の組み合わせでもTG量に有意差はなかった。TG量・R1量は、いずれの器具および従来法においてもR1量が有意に低い値を示した。R1量では、OB/LBCはIB・CS/従来法に比べ、有意に高い値を示した。IB/LBCはCS/従来法に比べ、有意に高い値を示した。OB/従来法はCS/従来法に比べ、有意に高い値を示した。ブラシ系器具を用いたLBC法は標本の細胞回収に貢献し、細胞量不足による不適正標本の防止および精度管理の向上に繋が ることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"We compared factors affecting the quality of oral mucosal cells recovered by scraping cytology. Collection devices were compared using device observations, and preparation methods were compared using quantitative analysis of recovered cells. In five healthy volunteers, the left lingual margin was stained with a plaque-staining solution, and cells were collected using an Orcellex brush® (OB), interdental brush (IB), and cotton swab (CS). The device surfaces were observed under a stereomicroscope during specimen preparation by conventional method (CM) and liquid-based cytology (LBC). The total collection volumes of genomic DNA (TG) in the volume recovered (on the glass slides and in the LBC preservation solution R1) were statistically compared for each device. Although the condition of cell harvesting differed among the devices, there was no significant change in the volume of TG in any combination of sampling devices and preparation methods. The TG and R1 contents were significantly lower for R1 in both sampling devices and the conventional method. The R1 values of OB/LBC were significantly higher than those of IB and CS/conventional methods; IB/LBC were significantly higher than those of CS/conventional methods; and OB/conventional methods were significantly higher than CS/conventional methods. The LBC using a brush-based collection device contributes to cell collection and prevents inappropriate specimens due to insufficient cell volume, suggesting that the method can improve accuracy control.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10001_textarea_24":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"朝日大学"}]},"item_10001_textarea_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"岐阜歯科学会"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"朝日大学大学院歯学研究科口腔病理学専攻","affiliationNameLang":"ja"},{"affiliationName":"D.D.S, Department of Oral Pathology, Asahi University Graduate School of Dentistry","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"松岡, 太相","creatorNameLang":"ja"},{"creatorName":"MATSUOKA, TAISO","creatorNameLang":"en"}],"familyNames":[{"familyName":"松岡","familyNameLang":"ja"},{"familyName":"MATSUOKA","familyNameLang":"en"}],"givenNames":[{"givenName":"太相","givenNameLang":"ja"},{"givenName":"TAISO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"16902","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-12-02"}],"filename":"gifushika501_3948_2023.pdf","filesize":[{"value":"1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://asahi-u.repo.nii.ac.jp/record/2000077/files/gifushika501_3948_2023.pdf"},"version_id":"0a370f16-2163-4c3c-91a0-35d6e295f1ca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"口腔擦過細胞診","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"採取器具","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"採取量","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Oral scraping cytology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Collection device","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Collected volume","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"口腔粘膜擦過細胞診における細胞採取器具と標本作製法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"口腔粘膜擦過細胞診における細胞採取器具と標本作製法の検討","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"21","path":["1694594900953"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-12-02"},"publish_date":"2023-12-02","publish_status":"0","recid":"2000077","relation_version_is_last":true,"title":["口腔粘膜擦過細胞診における細胞採取器具と標本作製法の検討"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-05-08T02:46:02.859577+00:00"}