{"created":"2023-06-23T12:14:03.208919+00:00","id":2361,"links":{},"metadata":{"_buckets":{"deposit":"2b198aad-1329-4bd3-bb0e-34c1b60c3431"},"_deposit":{"created_by":19,"id":"2361","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"2361"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00002361","sets":["64:123"]},"author_link":["2820"],"item_10_creator_30":{"attribute_name":"教員氏名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹村, 元三"}],"nameIdentifiers":[{"nameIdentifier":"2820","nameIdentifierScheme":"WEKO"}]}]},"item_10_date_36":{"attribute_name":"発行又は発表の年月","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-02-05"}]},"item_10_text_41":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_text_value":"24374197"}]},"item_10_textarea_29":{"attribute_name":"業績分類","attribute_value_mlt":[{"subitem_textarea_value":"学術雑誌論文"}]},"item_10_textarea_31":{"attribute_name":"発行、発表雑誌等、又は発表学会等の名称","attribute_value_mlt":[{"subitem_textarea_value":"Eur J Pharmacol."}]},"item_10_textarea_32":{"attribute_name":"巻","attribute_value_mlt":[{"subitem_textarea_value":"724"}]},"item_10_textarea_34":{"attribute_name":"掲載ページ","attribute_value_mlt":[{"subitem_textarea_value":"58-66"}]},"item_10_textarea_35":{"attribute_name":"単著、共著の別","attribute_value_mlt":[{"subitem_textarea_value":"共著"}]},"item_10_textarea_42":{"attribute_name":"概要","attribute_value_mlt":[{"subitem_textarea_value":"Sevoflurane preconditioning against myocardial ischemia-reperfusion injury is lost if the ischemic insult is too long. Emerging evidence suggests that induction of autophagy may also confer cardioprotection against ischemia-reperfusion injury. We examined whether induction of autophagy prolongs sevoflurane preconditioning protection during a longer ischemic insult. Isolated guinea pigs hearts were subjected to 30 or 45 min ischemia, followed by 120 min reperfusion (control). Anesthetic preconditioning was elicited with 2% sevoflurane for 10 min prior to ischemia (SEVO-30, SEVO-45). Chloramphenicol (autophagy upregulator, 300 µM) was administered starting 20 min before ischemia and throughout reperfusion in SEVO-45 (SEVO-45+CAP). To inhibit autophagy, 3-methyladenine (10 μM) was administered during sevoflurane administration in SEVO-45+CAP. Infarct size was determined by triphenyltetrazolium chloride stain. Tissue samples were obtained before ischemia to determine autophagy-related protein (microtubule-associated protein light chain I and II: LC3-I, II), Akt and glycogen synthase kinase 3β (GSK3β) expression using Western blot analysis. The effect of autophagy on calcium-induced mitochondrial permeability transition pore (MPTP) opening in isolated calcein-loaded mitochondria was assessed. Electron microscopy was used to detect autophagosomes. Infarct size was significantly reduced in SEVO-30, but not in SEVO-45. Chloramphenicol restored sevoflurane preconditioning lost by 45 min ischemia. There were more abundant autophagozomes and LC3-II expression was significantly increased in SEVO-45+CAP. Induction of autophagy before ischemia enhanced GSK3β phosphorylation and inhibition of calcium-induced MPTP opening. These effects were abolished by 3-methyladenine. Pre-ischemic induction of autophagy restores sevoflurane preconditioning lost by longer ischemic insult. This effect is associated with enhanced inhibition of MPTP by autophagy."}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"other","resourceuri":"http://purl.org/coar/resource_type/c_1843"}]},"item_title":"Induction of autophagy restores the loss of sevoflurane cardiac preconditioning seen with prolonged ischemic insult.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Induction of autophagy restores the loss of sevoflurane cardiac preconditioning seen with prolonged ischemic insult."}]},"item_type_id":"10","owner":"19","path":["123"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-02-20"},"publish_date":"2015-02-20","publish_status":"0","recid":"2361","relation_version_is_last":true,"title":["Induction of autophagy restores the loss of sevoflurane cardiac preconditioning seen with prolonged ischemic insult."],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-06-23T14:55:10.300555+00:00"}