{"created":"2023-06-23T12:14:55.066523+00:00","id":3531,"links":{},"metadata":{"_buckets":{"deposit":"d5c00df2-02e9-4eda-b891-992b0bee006f"},"_deposit":{"created_by":21,"id":"3531","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"3531"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00003531","sets":["46:190:203"]},"author_link":["4093","4091","508","549","4092"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"120","bibliographicPageStart":"108","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"リン酸カルシウム基材の吸収と新生骨の形成の関係をWistar系ラットの大腿骨に各種リン酸カルシウム基材を填入する動物実験を行って、特に、これらの基材の吸収と新生骨の形成に対する破骨細胞の関わりとその破骨細胞の分化に対する破骨細胞分化因子(RANKL)とそのレセプターのRANKの関係を検討した。実験は何ら処置を施さない無処置群、2×3×2mmの骨欠損の形成のみの欠損群、形成後β-TCPを填入したTCP群、750℃で2時間焼結したCAPを填入した焼結CAP群、非焼結のCAPを填入した非焼結CAP群の5群に分けて3日～2ヵ月後の経時的変化をHE染色、TRAP染色、抗RANK抗体と抗RANKL抗体を用いた免疫染色によって観察を行った。その結果、各基材の群は填入後、3日および5日に、填入部に肉芽組織が増殖し、そこから骨芽細胞の分化、類骨形成、新生骨形成を認めた。TCP群と焼結CAP群は新生骨形成までの期間が長く、非焼結CAP群は短かった。また、TRAP陽性破骨細胞の動態は基材の吸収だけでなく新生骨の吸収にも見られ、特にCAPの吸収は非焼結CAP群で顕著であった。さらに、RANKとRANKLの免疫染色レベルにおける発現は、TRAP陽性破骨細胞がRANK陽性細胞と一致し、破骨細胞への分化を誘導するRANKL陽性細胞は無処置群の正常状態では、骨髄細胞中に多く存在し、形状が一定でない成熟度の低い幹細胞であることが示唆された。また、その骨髄組織にはRANK陽性細胞はほとんどなかった。一方、欠損群ではそこに増殖した肉芽組織中にRANKL陽性細胞が出現し、これに伴い、肉芽組織中に形成された類骨周囲にRANK陽性細胞の増加を認めるようになった。その後、肉芽組織のRANK陽性細胞は減少傾向を示し、RANKL陽性細胞の由来は不明であった。TCP群と焼結CAP群は填入後、3日～2ヵ月間でRANKL陽性細胞を認め、RANK陽性細胞ではTCP群が填入後、5日と1週間のみで認められ、焼結CAP群は3日では認められず、それ以降で観察できた。非焼結CAP群は填入後、3日～1週間まではRANKL陽性細胞を認め、それ以降では認められず、RANK陽性細胞は填入後1週間後を除いてすべての期間で観察された。これらのことからリン酸カルシウム基材をWistar系ラットの大腿骨に適用した場合、各リン酸カルシウム基材の種類によってRANKおよびRANKL陽性細胞の経時的動態は異なり、これらの基材の吸収ならびに新生骨の形成にはRANKおよびRANKL陽性細胞が深く関与していることが示唆された。これらリン酸カルシウム基材には、一定期間内は補強材として、その後、新生骨の形成とともに消失し、骨と置換する機能を有する骨補填材としてのさらなる改良が望まれた。","subitem_description_type":"Abstract"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"508","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000250763990","nameIdentifierScheme":"CiNii 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Phosphates","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Receptor Activator of Nuclear Factor-Kappa B","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Beta-Tricalcium Phosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carboapatite","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"リン酸カルシウム基材填入によるRANKおよびRANKL陽性細胞の発現の動態","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リン酸カルシウム基材填入によるRANKおよびRANKL陽性細胞の発現の動態"},{"subitem_title":"Temporal and Time-spatial Expression of RANK and RANKL in the Bone Marrow Cells Induced by Calcium Phosphate Base 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