{"created":"2023-06-23T12:15:26.876647+00:00","id":5186,"links":{},"metadata":{"_buckets":{"deposit":"3067d443-c1ae-490d-82d2-2d8b87a128f2"},"_deposit":{"created_by":21,"id":"5186","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"5186"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00005186","sets":["46:190:346"]},"author_link":["8726","8724","8727","345","8728","535","6889"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"66","bibliographicPageStart":"61","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ラット臼歯のエナメル芽細胞が大量のCa2+を司る機構、Ca2+の動員、Ca2+のシグナル伝達分子の局在性を明らかにするため、Ca2+結合タンパクであるCalmodulin(CaM),Calbindin(CaB)とチロシンキナーゼ型レセプターであるPDGF-R,FGF-R,PLC-γ、およびCa2+維持機構に関わるIP3-RとCa-ATPaseの免疫組織化学的局在性をラット臼歯で検討した。CaBは分化期、分泌期では強い局在性を示し、成熟期ではエナメル芽細胞内中央部より遠心側に強い局在性を認めた。CaBは緩衝型タンパクと呼ばれ細胞内のCa2+濃度調節に関わることから、CaBが分化期、分泌期、成熟期エナメル芽細胞のCa2+濃度調節を担っていることが示唆された。CaMは分化期、分泌期では弱く、成熟期および隣接する中間層細胞、乳頭層細胞に強い局在性を示した。Ca-ATPaseはCaMとほぼ同様の局在性を示した。CaMはCa2+と結合後、標的蛋白質と結合して生理機能を発揮するとされることから、CaMはエナメル芽細胞の細胞機能に関わることが推察され、Ca-ATPaseはCaM依存性酵素であることから、CaMは成熟期エナメル芽細胞および乳頭層細胞のCa2+排出に関わることが示唆された。PDGF-R,FGF-R,PLC-γは、分化期、分泌期に強い局在がみられ、乳頭層細胞に強い局在がみられた。IP3-Rは分化期、分泌期、分泌期後期にもっとも強い局在性を示した。レセプターからのPIレスポンスによるCa2+動員は、分化期、分泌期に、最も活発なことが示唆された。以上のことから、エナメル芽細胞内のCa2+動態は細胞の機能発現に関わるシグナルと、成熟期エナメル芽細胞および中間層細胞、乳頭層細胞内では機能発現のシグナルと石灰化のための輸送に関与していることが示唆された。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The immunohistochemical localization of calmodulin( CaM)and calbindin( CaB), which are Ca^2+ -binding proteins,platelet-derived endothelial growth factor-receptor( PDGF-R), fibroblast growth factor-receptor( FGF-R),and PLC-γ, which are tyrosine kinase receptors, and IP3-R and Ca-ATPase, which are involved in Ca^2+ maintenance,were evaluated in rat molars to clarify the mechanism by which ameloblasts in rat molars control large levels of Ca^2+ ,the mobilization of Ca^2+ , and the localization of Ca^2+ -signaling molecules.CaB showed strong localization in differentiation and secretory stages, and stronger localization on the distal side ofthe ameloblasts in the maturation stage. CaB is referred to as a buffer protein, and is involved in adjusting the Ca^2+concentration; therefore, it was suggested that CaB is related to adjustment of the Ca^2+ concentration in theameloblasts in differentiation, secretory and maturation stages.CaM showed weak localization in differentiation and secretory stages, and strong localization in the maturation stage and on the interlayer and stratum papillary cells. Ca-ATPase revealed a similar localization pattern to that ofCaM. CaM binds to Ca^2+ , and then to a target protein, and becomes physiologically active; therefore, it was speculatedto be involved in the cellular functions of ameloblasts. Ca-ATPase was suggested to be involved in the excretionof Ca^2+ by ameloblasts and stratum papillary cells in the maturation period, because it is a CaM-dependent enzyme.PDGF-R, FGF-R, and PLC-γshowed strong localization in differentiation and secretory stages and on the stratumpapillary cells. IP3-R showed marked localization in differentiation and secretory stages, especially in the late phaseof secretion. The mobilization of Ca^2+ through the PI response involving the receptors was suggested to be the mostactive in differentiation and secretory stages. It was suggested that Ca^2+ dynamics in ameloblasts are associated withsignals involving expressions of cellular function, and Ca^2+ dynamics in interlayer and stratum papillary cells are associatedwith signals involving the expression of function and transportation for calcification.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"6889","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YAO, JUN"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"朝日大学歯学部","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"多賀谷","familyNameLang":"ja"},{"familyName":"TAGAYA","familyNameLang":"en"}],"givenNames":[{"givenName":"正俊","givenNameLang":"ja"},{"givenName":"MASATOSHI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8724","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004592896","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004592896"}],"names":[{"name":"多賀谷, 正俊","nameLang":"ja"},{"name":"TAGAYA, MASATOSHI","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"8728","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TANASE, SEIZO"}]},{"nameIdentifiers":[{"nameIdentifier":"345","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAMURA, YASUO"}]}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110007491610","subitem_relation_type_select":"NAID"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"福建医科大学附属口腔医科儿童牙科\nDepartment of Pediatric Dentistry, Fujian University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座小児歯科学分野\nDepartment of Pediatric Dentistry, Division of Oral Structure, Function and Development Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座小児歯科学分野\nDepartment of Pediatric Dentistry, Division of Oral Structure, Function and Development Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座小児歯科学分野\nDepartment of Pediatric Dentistry, Division of Oral Structure, Function and Development Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"姚, 軍"}],"nameIdentifiers":[{"nameIdentifier":"8726","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000014650569","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000014650569"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"多賀谷, 正俊","creatorNameLang":"ja"},{"creatorName":"TAGAYA, MASATOSHI","creatorNameLang":"en"}],"familyNames":[{"familyName":"多賀谷","familyNameLang":"ja"},{"familyName":"TAGAYA","familyNameLang":"en"}],"givenNames":[{"givenName":"正俊","givenNameLang":"ja"},{"givenName":"MASATOSHI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8724","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004592896","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004592896"}]},{"creatorNames":[{"creatorName":"棚瀬, 精三"}],"nameIdentifiers":[{"nameIdentifier":"8727","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004593016","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004593016"}]},{"creatorNames":[{"creatorName":"田村, 康夫"}],"nameIdentifiers":[{"nameIdentifier":"535","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000040113047","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000040113047"},{"nameIdentifier":"40113047","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40113047"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-01-23"}],"displaytype":"detail","filename":"gifushika352_6166_2008.pdf","filesize":[{"value":"192.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika352_6166_2008","url":"https://asahi-u.repo.nii.ac.jp/record/5186/files/gifushika352_6166_2008.pdf"},"version_id":"76aa7aa2-635a-4148-8c81-ea6c26c562e0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"カルモデュリン","subitem_subject_scheme":"Other"},{"subitem_subject":"カルビンディン","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット臼歯","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメル形成","subitem_subject_scheme":"Other"},{"subitem_subject":"線維芽細胞増殖因子","subitem_subject_scheme":"Other"},{"subitem_subject":"血小板由来増殖因子","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメル質形成","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫組織化学","subitem_subject_scheme":"Other"},{"subitem_subject":"臼歯","subitem_subject_scheme":"Other"},{"subitem_subject":"Sprague-Dawleyラット","subitem_subject_scheme":"Other"},{"subitem_subject":"カルシウムシグナル伝達","subitem_subject_scheme":"Other"},{"subitem_subject":"Calmodulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rat molars","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Amelogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium-Transporting ATPases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Calcium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Inositol 1,4,5-Trisphosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase C Gamma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Calbindins","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ラット臼歯エナメル質形成におけるCa2+結合性タンパクおよび細胞内Ca2+シグナル伝達分子の免疫組織化学的局在性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ラット臼歯エナメル質形成におけるCa2+結合性タンパクおよび細胞内Ca2+シグナル伝達分子の免疫組織化学的局在性"},{"subitem_title":"The Immunohistochemical Localization of Ca2+-binding Protein and Intracellular Ca2+-signaling Molecules during Amelogenesis in Rat Molars","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["346"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-01-23"},"publish_date":"2017-01-23","publish_status":"0","recid":"5186","relation_version_is_last":true,"title":["ラット臼歯エナメル質形成におけるCa2+結合性タンパクおよび細胞内Ca2+シグナル伝達分子の免疫組織化学的局在性"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-01-16T05:33:21.132775+00:00"}