{"created":"2023-06-23T12:15:28.723549+00:00","id":5228,"links":{},"metadata":{"_buckets":{"deposit":"f7e1b55a-180f-4037-a090-4cd381ffc754"},"_deposit":{"created_by":21,"id":"5228","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"5228"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00005228","sets":["46:190:351"]},"author_link":["8629","8734","8627","6806","8732","5691","8624","8731","8625","8733"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"98","bibliographicPageStart":"93","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ラット3大唾液腺(耳下腺、顎下腺、舌下腺)および肝由来生体膜の物理化学的性質を電子スピン共鳴(ESR)法で比較検討した。ミクロソーム画分では耳下腺、肝が顎下腺、舌下腺より膜流動性に富んでいた。耳下腺ホモジェネートから、遠心分画、MnCl2処理、Percoll密度勾配遠心分画の組合せで調製した細胞内小器官の生体膜の物理化学的性質を検討したところ、ミトコンドリア画分が小胞体画分、リソソーム含有ミトコンドリア画分より膜流動性に富んでいた。さらに、膜融合の結果惹起される唾液分泌に関与する細胞内小器官(分泌顆粒と頂端側形質膜)さらに小胞体の膜物性を流動性で検討すると、分泌顆粒>小胞体>頂端側形質膜の順に増加した。これらの物性は、細胞内小器官に特有の生体膜構成脂質のみならず、各小器官特有の機能を発揮する膜タンパク質の物理化学的性質を反映していると考えられる。さらに、耳下腺細胞のように細胞極性を持つ分泌細胞の頂端側形質膜にはラフト様構造の存在が指摘されている。これらの結果は、耳下腺唾液の分泌時には、膜流動性に富む分泌顆粒が流動性に乏しく、硬い頂端側形質膜と融合することによって部分的に形質膜を脆弱化し、膜融合後の外分泌を惹起していることが示唆された。","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"In order to clarify differences in the physical properties of native membranes in the rat salivary glands (parotid, submandibular and sublingual glands), the fluidity of their microsomal membrane fractions was observed by electron spin resonance study. Microsomes from the parotid gland showed a higher membrane fluidity than those of the submandibular and sublingual glands. To distinguish the specific characteristics of subcellar membrane physical properties,mitochondria-, endoplasmic reticulum-, apical plasma membrane- and secretory granule-rich fractions were purified from parotid glands. The order parameter of the mitochondria-rich fraction is smaller than that of the endoplasmic reticulum-rich fraction, indicating that mitochondrial membranes are more fluid than endoplasmic reticular membranes. However, the secretory granule-rich fraction of native membranes also showed more fluid than those from endoplasmic reticulum-rich fraction. The physical property of the apical plasma membranes, which is the fusion site for the saliva secretion, were the opposite of those of the secretory granules and the fluidity of the apical plasma membrane-rich fraction was more rigid than that of endoplasmic reticulum-rich fraction. In polarized cells such as those of the parotid gland, the apical plasma membranes demonstrate lipid raft-like structures. These specific membrane domains may affect the physical properties of the membrane and control the stability of the apical plasma membranes. These findings suggest that in the steps of saliva secretion, the fluid membranes supplied from secretary granules may disturb the rigid apical plasma membranes after fusion and enhance exocytosis.","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"5691","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMEYAMA, YASUNAGA"}]},{"nameIdentifiers":[{"nameIdentifier":"8733","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KITAMURA, KEISHI"}]},{"nameIdentifiers":[{"nameIdentifier":"6806","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YASHIRO, KOJI"}]},{"nameIdentifiers":[{"nameIdentifier":"8629","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMIYA, MASAKO"}]},{"nameIdentifiers":[{"nameIdentifier":"8734","nameIdentifierScheme":"WEKO"}],"names":[{"name":"FUJITA, ATSUSHI"}]}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_relation_85":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110007405481","subitem_relation_type_select":"NAID"}}]},"item_10002_relation_86":{"attribute_name":"医中誌文献番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"2008166296","subitem_relation_type_select":"ICHUSHI"}}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0072","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔生化学分野\nDepartment of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔生化学分野\nDepartment of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔生化学分野\nDepartment of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔生化学分野\nDepartment of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔構造機能発育学講座口腔生化学分野\nDepartment of Oral Biochemistry, Division of Oral Structure, Function and Development, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"亀山, 泰永"}],"nameIdentifiers":[{"nameIdentifier":"8624","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050161245","nameIdentifierScheme":"NRID","nameIdentifierURI":" https://rns.nii.ac.jp/d/nr/1000050161245"}]},{"creatorNames":[{"creatorName":"北村, 圭司"}],"nameIdentifiers":[{"nameIdentifier":"8731","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004730873","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004730873"}]},{"creatorNames":[{"creatorName":"八代, 耕児"}],"nameIdentifiers":[{"nameIdentifier":"8625","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050182316","nameIdentifierScheme":"NRID","nameIdentifierURI":" https://rns.nii.ac.jp/d/nr/1000050182316"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"33703","affiliationNameIdentifierScheme":"kakenhi","affiliationNameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=33703"}],"affiliationNames":[{"affiliationName":"朝日大学","affiliationNameLang":"ja"},{"affiliationName":"Chemistry Laboratory, Department of Management and Information studies, Asahi University School of Business Administration","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"神谷, 真子","creatorNameLang":"ja"},{"creatorName":"KAMIYA, MASAKO","creatorNameLang":"en"}],"familyNames":[{"familyName":"神谷","familyNameLang":"ja"},{"familyName":"KAMIYA","familyNameLang":"en"}],"givenNames":[{"givenName":"真子","givenNameLang":"ja"},{"givenName":"MASAKO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8627","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000080181907","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000080181907"},{"nameIdentifier":"80181907","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80181907"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"60067098","affiliationNameIdentifierScheme":"kakenhi","affiliationNameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60067098"}],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藤田, 厚","creatorNameLang":"ja"},{"creatorName":"Fujita, Atsushi","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤田","familyNameLang":"ja"},{"familyName":"Fujita","familyNameLang":"en"}],"givenNames":[{"givenName":"厚","givenNameLang":"ja"},{"givenName":"Atsushi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"8732","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000060067098","nameIdentifierScheme":"NRID","nameIdentifierURI":"https://rns.nii.ac.jp/d/nr/1000060067098"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-09"}],"displaytype":"detail","filename":"gifushika343_9398_2008.pdf","filesize":[{"value":"630.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika343_9398_2008","url":"https://asahi-u.repo.nii.ac.jp/record/5228/files/gifushika343_9398_2008.pdf"},"version_id":"aa49c424-e3cd-4478-aee2-98fbc4edd498"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞分画","subitem_subject_scheme":"Other"},{"subitem_subject":"膜流動性","subitem_subject_scheme":"Other"},{"subitem_subject":"頂端側形質膜","subitem_subject_scheme":"Other"},{"subitem_subject":"分泌顆粒","subitem_subject_scheme":"Other"},{"subitem_subject":"顎下腺","subitem_subject_scheme":"Other"},{"subitem_subject":"耳下腺","subitem_subject_scheme":"Other"},{"subitem_subject":"舌下腺","subitem_subject_scheme":"Other"},{"subitem_subject":"電子スピン共鳴","subitem_subject_scheme":"Other"},{"subitem_subject":"生体膜","subitem_subject_scheme":"Other"},{"subitem_subject":"肝ミクロソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"Wistarラット","subitem_subject_scheme":"Other"},{"subitem_subject":"分泌小胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ラット","subitem_subject_scheme":"Other"},{"subitem_subject":"subcellar fractionation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"membrane fluidity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apical plasma membranes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"secretory granules","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rat parotid gland","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ラット唾液腺由来細胞内オルガネラの膜物性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ラット唾液腺由来細胞内オルガネラの膜物性"},{"subitem_title":"Physical Properties of Subcellular Membrane Fractions Isolated from Rat Salivary Glands","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["351"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-02-09"},"publish_date":"2017-02-09","publish_status":"0","recid":"5228","relation_version_is_last":true,"title":["ラット唾液腺由来細胞内オルガネラの膜物性"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-05-13T06:17:10.519995+00:00"}