{"created":"2023-06-23T12:15:32.637020+00:00","id":5314,"links":{},"metadata":{"_buckets":{"deposit":"86d92c42-f6c0-4486-b398-c5bc6349919f"},"_deposit":{"created_by":21,"id":"5314","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"5314"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00005314","sets":["46:348:357"]},"author_link":["4128","4071","3970","4126","340","4134","492","3802","4159","3971","4276","8844","4120","3972","4277"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"97","bibliographicPageStart":"93","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The goal of periodontal treatment may be recognized regeneration of periodontal tissue. The regeneration is composed cells, growth factors and scaffolds. This study was to evaluate the alkaline phosphatase (ALP) activity of mesenchymal stem cell (MSC) cultured with bone morphgenic protein (BMP) or fibroblast growth factor (FGF). The MSC was cultured with BMP or FGF for up to three weeks. The cultured glass slides were stained for ALP. The area of ALP positive on the glasses was measured. The total area of ALP positive was the highest in BMP group among the groups. In FGF group, the positive area was lower than BMP group, but significantly higher than that in the control group. BMP and FGF can enhance the osteogenic activity for MSC. FGF can be a perdicatble material for periodontal regeneration. ","subitem_description_type":"Abstract"}]},"item_10002_description_81":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"間葉系幹細胞（MSC）は骨髄中に存在する体性幹細胞で、造血システムを維持するうえで重要な役割を果たしている。MSCは多彩な分化能を有し、生体内投与を行うと、組織傷害部位に集積する性質があることから再生医療への応用が期待されている。本研究は培養MSCに成長因子のBMPやFGFを添加することによってMSCのALP活性におよぼす影響を検討した。BMPまたはFGFを添加した培養液でガラススライド上に播種したMCSを培養した。培養終了後にALP染色を行いALP陽性面積を測定した。BMPはMSCの多くの報告と同様にALP活性を顕著に促進した。またFGFにおいてもBMPより低いものの、ALP活性を有意に増加させた。これらの結果はBMPと同様にFGFもMSCのALP活性を向上させる能力があり、歯周組織再生療法への応用が期待されると考えられる。","subitem_description_type":"Other"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"4276","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000342905810","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000342905810"}],"names":[{"name":"山岡, 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Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"},{"subitem_textarea_value":"Department of Periodontology, Devision of Oral Infection and Disease, Asahi University School of Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"},{"subitem_textarea_value":"Department of Periodontology, Devision of Oral Infection and Disease, Asahi University School of Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"},{"subitem_textarea_value":"Department of Periodontology, Devision of Oral Infection and Disease, Asahi University School of Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"},{"subitem_textarea_value":"Department of Periodontology, Devision of Oral Infection and Disease, Asahi University School of Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"},{"subitem_textarea_value":"Department of Endodontology, Division of Oral Functional Science and Rehabilitation, Asahi University School of Dentistry\n朝日大学歯学部口腔機能修復学講座保存学分野歯内療法学"},{"subitem_textarea_value":"Department of Dentistry for the Disability and Oral Health Division of Oral Pathogenesis and Control, Asahi University School of Dentistry\n朝日大学歯学部口腔病態医療学講座障害者歯科学分野"},{"subitem_textarea_value":"Department of Periodontology, Devision of Oral Infection and Disease, Asahi University School of Dentistry\n朝日大学歯学部口腔感染医療学講座歯周病学分野"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMAOKA, TAKAHIRO"}],"nameIdentifiers":[{"nameIdentifier":"4277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YASUDA, 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MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika433_9397_2017","url":"https://asahi-u.repo.nii.ac.jp/record/5314/files/gifushika433_9397_2017.pdf"},"version_id":"c4bc918a-4a0a-4705-a645-b0046403c9ee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"間葉系幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"線維芽細胞成長因子","subitem_subject_scheme":"Other"},{"subitem_subject":"骨造成因子","subitem_subject_scheme":"Other"},{"subitem_subject":"アルカリフォスファターゼ活性","subitem_subject_scheme":"Other"},{"subitem_subject":"mesenchymal stem cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fibroblast growth factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone morphogenic protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"alkaline phosphate activity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"BMPとFGFによる培養間葉系幹細胞のアルカリフォスファターゼ活性への影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"BMPとFGFによる培養間葉系幹細胞のアルカリフォスファターゼ活性への影響"},{"subitem_title":"BMP and FGF enhance alkaline phosphatase activity for rat mesenchymal stem cell","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["357"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-05-20"},"publish_date":"2017-05-20","publish_status":"0","recid":"5314","relation_version_is_last":true,"title":["BMPとFGFによる培養間葉系幹細胞のアルカリフォスファターゼ活性への影響"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-03-29T08:45:27.599561+00:00"}