{"created":"2023-06-23T12:18:59.134521+00:00","id":9791,"links":{},"metadata":{"_buckets":{"deposit":"80f8bfcd-03e2-433b-b94a-83a9be621d59"},"_deposit":{"created_by":21,"id":"9791","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"9791"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00009791","sets":["46:348:384"]},"author_link":["4107","3134","13275","13276","13277","4561","540","6099"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"20","bibliographicPageStart":"9","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"局所麻酔薬は神経細胞や心筋細胞のNa+チャネルを阻害して、神経細胞刺激伝導の抑制による局所麻酔作用や心筋細胞興奮の抑制による抗不整脈作用を発現する。また、局所麻酔薬はNa+チャネル以外の標的分子に作用することが知られているがそれらの詳細な機序については不明な点が多い。本研究で、我々はfMLPで惹起された好中球の活性酸素産生と遊走能に対する局所麻酔薬の効果について検討を行った。全ての局所麻酔薬（リドカイン、ブピバカインおよびプロカイン）はfMLPで惹起された好中球の活性酸素産生と遊走能を濃度依存的に抑制した。また、局所麻酔薬はfMLPによるp47phoxとPKCαの膜転移の膜転移、Aktとミオシン軽鎖のリン酸化を抑制した。カルシウム-カリモジュリン経路はp47phox、PKC、Aktおよびミオシン軽鎖のリン酸化に深く関わることが知られているが、本研究において局所麻酔薬はカルモジュリン依存性のphosphodiesteraseを抑制することがわかった。以上の結果から、局所麻酔薬は少なくともその一部がカルシウム-カリモジュリンのシグナル伝達を介して好中球の機能を抑制することが明らかになった。\nLocal anesthetics are known to block the function of Na+ channels in nerve and cardiac cells, and also to affect the other functions of many cells. However, the precise mechanisms of these functions are still unknown. In this experiment, we investigated the effects of local anesthetics on superoxide production and chemotaxis in fMLP-stimulated neutrophils. All three local anesthetics (lidocaine, bupivacaine, and procaine) suppressed superoxide production and chemotaxis in fMLP-stimulated neutrophils, in a concentration-dependent manner. These anesthetics also suppressed the fMLP-stimulated membrane translocations of p47phox and PKCα, phosphorylatios of Akt and myosin light chain of myosin II. It is well known that a calcium-calmodulin cascade mediates the phosphorylations of the above signaling molecules, and local anesthetics suppressed the calmodulin dependent activation of phosphodiesterase in vitro. These results suggest that local anesthetics at least partially suppress the activation of neutrophil functions through the calcium-calmodulin signal transduction system.","subitem_description_type":"Abstract"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"13276","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KASHI, SOSUKE"}]},{"nameIdentifiers":[{"nameIdentifier":"540","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003762491","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003762491"}],"names":[{"name":"AZUMA, YUKIO"}]},{"nameIdentifiers":[{"nameIdentifier":"13277","nameIdentifierScheme":"WEKO"}],"names":[{"name":"CHIHARA, EIICHI"}]},{"nameIdentifiers":[{"nameIdentifier":"4561","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KASHIMATA, MASANORI"}]}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部総合医科学講座麻酔学分野\nDepartment of Anesthesiology, Division of General Medicine, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infection and Health Sciences, Asahi University School of Dentistry "},{"subitem_textarea_value":"朝日大学歯学部総合医科学講座麻酔学分野\nDepartment of Anesthesiology, Division of General Medicine, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infection and Health Sciences, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"家始, 聡介"}],"nameIdentifiers":[{"nameIdentifier":"13275","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000258725833","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000258725833"}]},{"creatorNames":[{"creatorName":"東, 幸雄"}],"nameIdentifiers":[{"nameIdentifier":"6099","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003762491","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003762491"}]},{"creatorNames":[{"creatorName":"智原, 栄一"}],"nameIdentifiers":[{"nameIdentifier":"4107","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000250472703","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000250472703"},{"nameIdentifier":"1000080244581","nameIdentifierScheme":"NRID","nameIdentifierURI":" https://rns.nii.ac.jp/d/nr/1000080244581"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"柏俣, 正典","creatorNameLang":"ja"},{"creatorName":"KASHIMATA, MASANORI","creatorNameLang":"en"}],"familyNames":[{"familyName":"柏俣","familyNameLang":"ja"},{"familyName":"KASHIMATA","familyNameLang":"en"}],"givenNames":[{"givenName":"正典","givenNameLang":"ja"},{"givenName":"MASANORI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3134","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000030152630 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000030152630 "},{"nameIdentifier":"30152630","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30152630"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-08"}],"displaytype":"detail","filename":"gifushika451_920_2018.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika451_920_2018","url":"https://asahi-u.repo.nii.ac.jp/record/9791/files/gifushika451_920_2018.pdf"},"version_id":"cfa85158-cdf5-4cfa-8689-f38a358b9dde"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"好中球","subitem_subject_scheme":"Other"},{"subitem_subject":"シグナル伝達","subitem_subject_scheme":"Other"},{"subitem_subject":"局所麻酔薬","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophils","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"signal transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"local anesthetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"fMLPで誘導されるラット好中球の機能発現に及ぼす局所麻酔薬の影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"fMLPで誘導されるラット好中球の機能発現に及ぼす局所麻酔薬の影響"},{"subitem_title":"Effects of local anesthetics on superoxide production and chemotaxis in fMLP-stimulated rat neutrophils","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["384"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-07"},"publish_date":"2018-11-07","publish_status":"0","recid":"9791","relation_version_is_last":true,"title":["fMLPで誘導されるラット好中球の機能発現に及ぼす局所麻酔薬の影響"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2023-12-25T07:37:19.157655+00:00"}