{"created":"2023-06-23T12:18:59.177350+00:00","id":9792,"links":{},"metadata":{"_buckets":{"deposit":"dfdbcf95-7509-40b8-9b9f-53bfc4995560"},"_deposit":{"created_by":21,"id":"9792","owners":[21],"pid":{"revision_id":0,"type":"depid","value":"9792"},"status":"published"},"_oai":{"id":"oai:asahi-u.repo.nii.ac.jp:00009792","sets":["46:348:384"]},"author_link":["4064","3134","4561","3967","3998"],"item_10002_biblio_info_77":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"28","bibliographicPageStart":"21","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"岐阜歯科学会雑誌"},{"bibliographic_title":"The Journal of Gifu Dental Society","bibliographic_titleLang":"en"}]}]},"item_10002_description_80":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"　マウス顎下腺は胎生11日目（E11）に舌の基部に位置する上皮細胞と、 神経堤細胞由来の間葉細胞の相互作用によって上皮組織が肥厚し、間葉側へ陥入することから発生する。その後、上皮の先端は分枝と伸長を繰り返して、外分泌腺特有の枝分かれ構造を形成していく。この現象は分枝形態形成とよばれており、重要な器官形成機構の一つとしてよく知られている。Integrinは細胞外の情報を細胞内へ伝達（‘outside-in’ signal）するほか、細胞内の情報を細胞外へ伝達（‘inside-out’ signal）することで細胞の接着、分化、増殖、移動およびアポトーシスに関わることから上皮間葉相互作用を制御する因子と考えられている。本研究では、 顎下腺の分枝形態形成おけるintegrin α5の役割について検討を行った。すでに顎下腺原基に発現していることが知られている各種インテグリンサブユニット（α3，α5，α6，β1およびβ4サブユニット）のmRNAをリアルタイムRT-PCRにより定量解析した結果、顎下腺発生の初期（E13-E14）にα5サブユニットの発現が高いことが分かった。他のインテグリンサブユニットではβ1が初期で高値を示した。培養顎下腺原基に抗integrin α5抗体を添加すると5μg/mlの濃度以上で濃度依存的に分枝形態形成が抑制された。高濃度の抗integrin α5抗体（20μg/ml）では顎下腺原基の分枝形態形成はほぼ完全に抑制された。顎下腺上皮のみの培養系を用いた解析から、抗integrin α5抗体（10μg/ml）はEGFが誘導するcleft形成とFGF10が誘導するelongationを共に抑制した。抑制の程度はcleft形成で約20%、elongation形成で50%であった。また、抗integrin α5抗体の添加により、FGF10で惹起されるE13顎下腺原基のERK1/2のリン酸化の亢進が抑制された。\n　以上の結果から、integrin α5サブユニットは発生の初期段階で分枝形態形成に関わることが示唆された。また、integrin α5サブユニットは分枝形態形成を構成するcleft形成とelongationに共に関与しており、elongationにはERK1/2経路の活性化を介していると考えられた。\n Branching morphogenesis (BrM) is a developmental process by which many organs, such as the lungs, kidneys, and all exocrine glands, including the salivary glands, are formed. BrM is a dramatic biological event regulated by epithelial-mesenchymal interactions. The fundamental process of epithelial-mesenchymal interactions depends both on a variety of growth factors and their receptors and on the extracellular matrix (ECM) and integrins. The mouse submandibular gland (SMG) is a well-characterized model system for the study of epithelial-mesenchymal interactions involved in fetal organogenesis both in vivo and in vitro. There are several reports describing the molecular mechanisms of the BrM of SMG rudiments. For example, BrM requires both the mesenchyme and the epithelial basement membrane, and these components involve the interactions of both several integrins and their ligands in the ECM. In this study, we demonstrated the role of the integrin α5 subunit in the BrM of developing mouse SMG. The BrM of cultured E13 SMG was dose-dependently suppressed by administration of a blocking antibody for the integrin α5 subunit. The suppression of epithelial morphogenesis of SMG rudiments was also shown in mesenchyme-free epithelial culture system. These results suggest that the integrin α5 plays a critical role in the branching morphogenesis of fetal mouse SMG.","subitem_description_type":"Abstract"}]},"item_10002_full_name_75":{"attribute_name":"著者別名","attribute_value_mlt":[{"affiliations":[{"affiliationNames":[{"affiliationName":"朝日大学歯学部","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"赤井","familyNameLang":"ja"},{"familyName":"AKAI","familyNameLang":"en"}],"givenNames":[{"givenName":"崇浩","givenNameLang":"ja"},{"givenName":"TAKAHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4064","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000251017022","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000251017022"}],"names":[{"name":"赤井, 崇浩","nameLang":"ja"},{"name":"AKAI, TAKAHIRO","nameLang":"en"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"朝日大学","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"式守","familyNameLang":"ja"},{"familyName":"SHIKIMORI","familyNameLang":"en"}],"givenNames":[{"givenName":"道夫","givenNameLang":"ja"},{"givenName":"MICHIO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3967","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006648893","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006648893"},{"nameIdentifier":"70154193","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70154193"}],"names":[{"name":"式守, 道夫","nameLang":"ja"},{"name":"SHIKIMORI, MICHIO","nameLang":"en"}]},{"affiliations":[{"affiliationNames":[{"affiliationName":"","lang":"ja"}],"nameIdentifiers":[]}],"familyNames":[{"familyName":"住友","familyNameLang":"ja"},{"familyName":"SUMITOMO","familyNameLang":"en"}],"givenNames":[{"givenName":"伸一郎","givenNameLang":"ja"},{"givenName":"SHINICHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3998","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050216496","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050216496"},{"nameIdentifier":"50216496","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50216496"}],"names":[{"name":"住友, 伸一郎","nameLang":"ja"},{"name":"SUMITOMO, SHINICHIRO","nameLang":"en"}]},{"nameIdentifiers":[{"nameIdentifier":"4561","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KASHIMATA, MASANORI"}]}]},"item_10002_radio_89":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_radio_item":"application/pdf"}]},"item_10002_source_id_82":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433-0191","subitem_source_identifier_type":"ISSN"}]},"item_10002_source_id_84":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00053070","subitem_source_identifier_type":"NCID"}]},"item_10002_textarea_76":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔外科学分野\nDepartment of Oral and Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔外科学分野\nDepartment of Oral and Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔病態医療学講座口腔外科学分野\nDepartment of Oral and Maxillofacial Surgery, Division of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry"},{"subitem_textarea_value":"朝日大学歯学部口腔感染医療学講座歯科薬理学分野\nDepartment of Dental Pharmacology, Division of Oral Infections and Health, Asahi University School of Dentistry"}]},"item_10002_version_type_95":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"朝日大学歯学部","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"赤井, 崇浩","creatorNameLang":"ja"},{"creatorName":"AKAI, TAKAHIRO","creatorNameLang":"en"}],"familyNames":[{"familyName":"赤井","familyNameLang":"ja"},{"familyName":"AKAI","familyNameLang":"en"}],"givenNames":[{"givenName":"崇浩","givenNameLang":"ja"},{"givenName":"TAKAHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4064","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000251017022","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000251017022"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"朝日大学","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"式守, 道夫","creatorNameLang":"ja"},{"creatorName":"SHIKIMORI, MICHIO","creatorNameLang":"en"}],"familyNames":[{"familyName":"式守","familyNameLang":"ja"},{"familyName":"SHIKIMORI","familyNameLang":"en"}],"givenNames":[{"givenName":"道夫","givenNameLang":"ja"},{"givenName":"MICHIO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3967","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006648893","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006648893"},{"nameIdentifier":"70154193","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70154193"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"住友, 伸一郎","creatorNameLang":"ja"},{"creatorName":"SUMITOMO, SHINICHIRO","creatorNameLang":"en"}],"familyNames":[{"familyName":"住友","familyNameLang":"ja"},{"familyName":"SUMITOMO","familyNameLang":"en"}],"givenNames":[{"givenName":"伸一郎","givenNameLang":"ja"},{"givenName":"SHINICHIRO","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3998","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000050216496","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000050216496"},{"nameIdentifier":"50216496","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50216496"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"柏俣, 正典","creatorNameLang":"ja"},{"creatorName":"KASHIMATA, MASANORI","creatorNameLang":"en"}],"familyNames":[{"familyName":"柏俣","familyNameLang":"ja"},{"familyName":"KASHIMATA","familyNameLang":"en"}],"givenNames":[{"givenName":"正典","givenNameLang":"ja"},{"givenName":"MASANORI","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"3134","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000030152630 ","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000030152630 "},{"nameIdentifier":"30152630","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30152630"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-08"}],"displaytype":"detail","filename":"gifushika451_2128_2018.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"gifushika451_2128_2018","url":"https://asahi-u.repo.nii.ac.jp/record/9792/files/gifushika451_2128_2018.pdf"},"version_id":"7885e910-5e48-45db-8594-828204a168e6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"分枝形態形成","subitem_subject_scheme":"Other"},{"subitem_subject":"インテグリン","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞成長因子","subitem_subject_scheme":"Other"},{"subitem_subject":"情報伝達","subitem_subject_scheme":"Other"},{"subitem_subject":"branching morphogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"integrin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell growth factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"signal transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"胎仔マウス顎下腺の分枝形態形成におけるintegrin α5サブユニットの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎仔マウス顎下腺の分枝形態形成におけるintegrin α5サブユニットの役割"},{"subitem_title":"Role of integrin α5 subunit in branching morphogenesis in fetal mouse","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"21","path":["384"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-07"},"publish_date":"2018-11-07","publish_status":"0","recid":"9792","relation_version_is_last":true,"title":["胎仔マウス顎下腺の分枝形態形成におけるintegrin α5サブユニットの役割"],"weko_creator_id":"21","weko_shared_id":-1},"updated":"2024-01-15T06:05:52.803828+00:00"}