@article{oai:asahi-u.repo.nii.ac.jp:00009806,
 author = {駒形, 香菜子 and 藤原, 敦 and 渡邉, 竜太 and 江尻, 貞一 and EJIRI, SADAKAZU and 北井, 則行 and KITAI, NORIYUKI},
 issue = {2018-10},
 journal = {2018-10},
 month = {2018-10, 2018-11-09},
 note = {ラット上顎臼歯間に挿入したゴムを除去することで、メカニカルストレスを変動させ、それに伴う歯槽骨骨細胞のsclerostin産生能の変化を検索し、メカニカルストレス変動に対する骨細胞の反応機序の解明を試みた。8週齢雄性Wistar（Slc）ラット32匹を用いた。実験群は3群8匹ずつに分け、未処置群は8匹とした。上顎第一、第二臼歯間にゴムを24時間挿入後、ただちにト殺した24時間装着群、ゴムを除去して6時間後の除去後6時間群、24時間後の除去後24時間群、および未処置群を作製し、μCT撮影後、SOSTmRNAに対するin situ Hybridizationとsclerostinに対する免疫染色を行った。観察部位は第一臼歯近心根と近心口蓋根間の根間中隔歯槽骨（M-MP）および近心口蓋根と遠心口蓋根間の根間中隔歯槽骨（MP-DP）とした。未処置群のM-MP、MP-DPでは、SOSTmRNAとsclerostinが認められた。24時間装着群のM-MP、MP-DPでは、SOSTmRNAもsclerostinも認められなかった。除去後6時間群では、M-MPでSOSTmRNAとsclerostinが認められたが、MP-DP ではSOSTmRNAもsclerostinも認められなかった。除去24時間群のM-MP、MP-DPでは、SOSTmRNAとsclerostinが認められた。部位によってsclerostin産生能回復時間が異なる原因として、メカニカルストレスの強度またはメカニカルストレスに対する骨細胞の感受性が部位により異なることが考えられる。メカニカルストレスを加えることにより消失した骨細胞のsclerostin産生分泌能は、メカニカルストレス除去後6時間で回復し、SOSTmRNAの発現と同時期に骨基質中にsclerostinが分泌されることと部位によって骨細胞のsclerostin産生能回復時間が異なることが示された。
By removing the rubber inserted between the maxillary molars of rats, the mechanical stress was changed, and the accompanying change in the sclerostin production ability of alveolar bone osteocytes was assessed to clarify the mechanism underlying the osteocytes response to changes in mechanical stress. Thirtytwo 8-week-old male Wistar (Slc) rats were used. Experimental groups were divided into three treatment groups of eight animals each with the remaining eight animals in an untreated group. The groups were as follows : a group in which the rubber was inserted between the first and second molar teeth for 24h, after which the animals were immediately sacrificed (24h wearing group) ; two groups in which the rubber was inserted between the first and second molar teeth for 24h, after which the rubber was removed, the animals were sacrificed 6h after removal treatment (6h after group), and sacrificed 24h after removal treatment (24h after group). After micro-computed tomography imaging, SOSTmRNA in situ hybridization and sclerostin immunostaining were performed. The observation site was the alveolarbone of intraradicular septum between the first molar tooth mesial root and the mesial palatal root (M-MP), and the bone of intraradicular septum between the mesial palate root and the distal palatal root (MP-DP). In the untreated group, both SOSTmRNA expression and sclerostin staining were observed in the M-MP and MP-DP. In contrast, neither SOSTmRNA expression nor sclerostin staining was observed in the M-MP or MP-DP in the 24 hour wearing group. Furthermore, while both SOSTmRNA expression and sclerostin staining were observed in the M-MP in the 6h after group, neither SOSTmRNA expression nor sclerostin staining was observed in the MP-DP. Both SOSTmRNA expression and sclerostin staining were observed in the M-MP and MP-DP in the 24h after group. Because of the different force or susceptibility of osteocytes on each site, the time required to produce sclerostin by osteocytes may be different. The sclerostin production ability of osteocytes that had been eliminated by the application of mechanical stress recovered at 6h after removal of mechanical stress, and sclerostin was once again secreted into the bone matrix at the almost same time as the expression of SOSTmRNA in osteocytes. The recovery time of sclerostin production was found to differ depending on the site.},
 pages = {2018-10--2018-10},
 title = {メカニカルストレスの変動に伴う歯槽骨骨細胞のsclerostin産生能の変化},
 volume = {2018-10},
 year = {}
}