@article{oai:asahi-u.repo.nii.ac.jp:00009808,
 author = {堀田, 敬史 and 金山, 圭一 and KANAYAMA, KEIICHI and 竹内, 浩子 and TAKEUCHI, HIROKO and 森永, 啓嗣 and MORINAGA, HIROTSUGU and 向井, 景祐 and MUKAI, KEISUKE and 木村, 洋子 and KIMURA, YOKO and 長谷川, 徹 and HASEGAWA, TORU and 北後, 光信 and KITAGO, MITSUNOBU and 小足, 周平 and OTARI, SHUHEI and 佐藤, 匠 and SATO, TAKUMI and 清水, 雄太 and SHIMIZU, YUTA and 伊神, 裕高 and IKAMI, HIROTAKA and 渋谷, 俊昭 and SHIBUTANI, TOSHIAKI},
 issue = {2018-10},
 journal = {2018-10},
 month = {2018-10, 2018-11-14},
 note = {マイクロアレイで、数10から100数種類の血中サイトカインを同時に網羅的に測定することが可能になった。そこで今回、歯周組織破壊により全身に及ぼす影響を検討する一助として、重度歯周炎患者の臨床指標と血中サイトカインとの関連を調べた。
朝日大学病院歯周病科を受診し、研究の趣旨を理解したうえで同意書に署名捺印を行い完全なインフォームドコンセントの得られた患者を被験者とした。本研究は朝日大学歯学部倫理委員会の承認を受けている（承認番号20064）。被験者は男性16名、女性10名。口腔内診査を受けたのち、10枚法デンタルX線写真、歯周組織検査（Probing Pocket Depth，Bleeding on probing，歯の動揺度）を行った。検査の結果、重度の慢性歯周炎あるいは侵襲性歯周炎と診断された18名、Perioグループ（平均年齢38.5歳　男性10名、女性8名）と健康な歯周組織と診断された8名、Healthyグループ（平均年齢29.5歳　男性6名、女性2名）の2グループに分けた。サイトカインはHuman Inflammation Antibody Array‒Membrane を用いて検出した。平均PPD（mm）と相関関係を認めたのはMIP-1β、ICAM-1、IL-6sR、TIMP-2.
PPD7mm以上部位の割合（％）と相関関係を認めたのはTIMP-2、IL-6sR、MIP-1β、ICAM-1。BOP（％）と相関関係を認めたのはMIP-1βであった。今回、相関関係を認めたサイトカインは歯周病の重症診断の一助となる可能性が示唆された。中でもMIP-1βは平均PPD、PPD7mm以上部位の割合、BOPと多くの歯周検査値との間に相関を認めた。MIP-1βは歯周炎の重症度を示すマーカーとして期待される。
 Microarrays now enable the simultaneous, exhaustive measurement of the levels of several dozens to over 100 different blood cytokines. We investigated the association between clinical indicators and blood cytokine levels in patients with severe periodontitis. 
 The subjects were patients examined in the Periodontics Clinic of Asahi University Hospital who provided written informed consent to participate in the study. This study was approved by the Ethics Committee of Asahi University (approval number 20064). The subjects comprised 16 men and 10 women. After an oral examination, dental radiography (10 images) and periodontal examination [probing pocket depth (PPD), bleeding on probing (BOP), and tooth mobility] were performed. On the basis of these test results, the subjects were divided into two groups : the Perio group comprising 18 subjects with severe chronic periodontitis or aggressive periodontitis (10 men and 8 women; mean age 38.5 years) and the Healthy group comprising 8 subjects with healthy periodontal tissue (6 men and 2 women; mean age 29.5 years). Cytokines were detected using the Human Inflammation Antibody Array-Membrane.
 Macrophage inflammatory protein-1β (MIP-1β), intercellular adhesion molecule-1 (ICAM-1), interleukin-6 soluble receptor (IL-6sR), and tissue inhibitor of metalloproteinase-2 (TIMP-2) were correlated with mean PPD (mm). TIMP-2, IL-6sR, MIP-1β, and ICAM-1 were also correlated with the percentage of sites with PPD⊇7mm. MIP-1β was correlated with BOP (%).
 Our results suggested that the cytokines found to be correlated with the periodontal examination score may contribute to the determination of periodontitis severity. In particular, MIP-1β was correlated with several test values for periodontitis : mean PPD, the percentage of sites with PPD⊇7mm, and BOP. MIP-1β may thus be a potential marker for periodontitis severity.},
 pages = {2018-10--2018-10},
 title = {重度歯周炎患者の血中サイトカインと歯周組織検査値との関係},
 volume = {2018-10},
 year = {}
}