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Lipid Peroxidation Inhibition by Perioperatively Used Drugs and Membrane Interaction as One of Their Possible Mechanisms
https://asahi-u.repo.nii.ac.jp/records/2113
https://asahi-u.repo.nii.ac.jp/records/21137cf4364b-ad8d-4117-8d3d-4f6b8f513a06
| Item type | 朝日大学 教育・研究業績(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-02-20 | |||||
| タイトル | ||||||
| タイトル | Lipid Peroxidation Inhibition by Perioperatively Used Drugs and Membrane Interaction as One of Their Possible Mechanisms | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
| 資源タイプ | other | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 業績分類 | ||||||
| 値 | 学会発表 | |||||
| 教員氏名 |
土屋, 博紀
× 土屋, 博紀 |
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| 発行、発表雑誌等、又は発表学会等の名称 | ||||||
| 値 | The 6th International Conference on Drug Discovery and Therapy, Dubai (UAE) | |||||
| 単著、共著の別 | ||||||
| 値 | 単独 | |||||
| 発行又は発表の年月 | ||||||
| 日付 | 2014-02 | |||||
| 概要 | ||||||
| 値 | Background: Oxidative stress, an imbalance between reactive oxygen species production and biological antioxidant defense, is induced by not only a wide range of diseases but also anesthesia and surgical trauma. In search of the drugs to reduce oxidative stress in the perioperative period, we studied the lipid peroxidation-inhibitory effects of structurally different drugs associated with surgery and one of their possible mechanisms.Methods: The lipid peroxidation-inhibitory effect was fluorometrically determined using diphenyl-1-pyrenylphosphine (DPPP)-incorporated liposomal membranes which were treated with 10-200 μM drugs and reference antioxidants, and then peroxidized with 20 μM peroxynitrite. The membrane interaction was analyzed by measuring the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH)-labeled biomimetic membranes after treating with drugs and reference antioxidants at 10 and 200 μM.Results: The tested drugs concentration-dependently inhibited peroxynitrite-induced peroxidation of membrane lipids as well as antioxidant α-tocopherol, quercetin and (–)-epigallocatechin-3-gallate. The inhibition at each 10 μM was greatest in propofol, followed by guaiacol, thiopental, thymol, phenol, midazolam, diazepam, lidocaine, eugenol, procaine, bupivacaine, ropivacaine, sevoflurane, ketamine, mepivacaine and prilocaine. All of these drugs and antioxidants interacted with biomimetic membranes consisting of phospholipids and cholesterol to modify the membrane fluidity, suggesting that the interactivity with membrane lipid bilayers is, at least in part, responsible for the lipid peroxidation-inhibitory effects of perioperatively used anesthetic, disinfectant and analgesic drugs.Conclusion: In addition to their inherent effects, propofol and other drugs to inhibit lipid peroxidation may be effective against perioperative oxidative stress. The membrane interactivity could be a guide for discovering novel antioxidant drugs. | |||||
