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nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride.
https://asahi-u.repo.nii.ac.jp/records/1273
https://asahi-u.repo.nii.ac.jp/records/1273ddb2153e-240e-48e6-83ac-5904fc27a31f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
10520295_87_485493_2012 (3.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-01-08 | |||||
| タイトル | ||||||
| タイトル | nergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surfaceEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride. | |||||
| タイトル | ||||||
| タイトル | Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | 3,3'-Diaminobenzidine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Antigens, Bacterial | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Bacterial Proteins | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Bacteroides | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Citraconic Anhydrides | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Heating | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Horseradish Peroxidase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Immunohistochemistry | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Membrane Glycoproteins | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Microscopy, Energy-Filtering Transmission Electron | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Microwaves | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Moriguchi, K
× Moriguchi, K× Mitamura, Y× Iwami, J× Hasegawa, Y× Higuchi, N× Murakami, Yukitaka× Maeda, H× Yoshimura, F× Nakamura, H× Ohno, N |
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| 所属 | ||||||
| Oral Anatomy, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Oral Pathology, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Endodontics, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Microbiology, School of Dentistry, Aichi-Gakuin University | ||||||
| 所属 | ||||||
| Endodontics, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Oral Microbiology, Division of Oral Infections and Health Sciences, Asahi University School of Dentistry | ||||||
| 所属 | ||||||
| Oral Pathology, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Microbiology, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Endodontics, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University | ||||||
| 所属 | ||||||
| Oral Anatomy, Research Institute of Advanced Oral Science, School of Dentistry, Aichi Gakuin University | ||||||
| 書誌情報 |
en : Biotechnic & histochemistry : official publication of the Biological Stain Commission 巻 87, 号 8, p. 485-493, 発行日 2012-01-01 |
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| 出版者 | ||||||
| Informa healthcare | ||||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane. We examined the relationship between high molecular weight proteins and the S-layer using electron microscopic immunolabeling with chemical fixation and an antigen retrieval procedure consisting of heating in a microwave oven or autoclave with citraconic anhydride. Immunogold particles were localized clearly at the outermost cell surface. We also used energy-filtering transmission electron microscopy (EFTEM) to visualize 3, 3'-diaminobenzidine tetrahydrochloride (DAB) reaction products after microwave antigen retrieval with 1% citraconic anhydride. The three-window method for electron spectroscopic images (ESI) of nitrogen by the EFTEM reflected the presence of moieties demonstrated by the DAB reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies instead of immunogold particles. The mapping patterns of net nitrogen were restricted to the outermost cell surface. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane. We examined the relationship between high molecular weight proteins and the S-layer using electron microscopic immunolabeling with chemical fixation and an antigen retrieval procedure consisting of heating in a microwave oven or autoclave with citraconic anhydride. Immunogold particles were localized clearly at the outermost cell surface. We also used energy-filtering transmission electron microscopy (EFTEM) to visualize 3, 3'-diaminobenzidine tetrahydrochloride (DAB) reaction products after microwave antigen retrieval with 1% citraconic anhydride. The three-window method for electron spectroscopic images (ESI) of nitrogen by the EFTEM reflected the presence of moieties demonstrated by the DAB reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies instead of immunogold particles. The mapping patterns of net nitrogen were restricted to the outermost cell surface. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1473-7760 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 22984898 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.3109/10520295.2012.687117 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://informahealthcare.com/doi/pdf/10.3109/10520295.2012.687117 | |||||
| 関連名称 | 出版社版 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
